Biolegend, 126501, Purified anti-mouse CD183 (CXCR3) Antibody, 50μg

CD183, also known as CXCR3, is a member of the C-X-C chemokine family, characterized by a pair of cysteine residues separated by a single amino acid. CXCR3 is a 38 kD seven pass transmembrane receptor coupled to G-protein. It mediates Ca 2+ mobilization and chemotaxis in response to C-X-C chemokines, such as IP10 (CXCL10), MIG (CXCL9), I-TAC (CXCL11) and PF4 (CXCL4). CXCR3 is expressed primarily on activiated T lymphocytes, NK cells, and some epithelial cells and endothelial cells. It is not expressed on B cells, monocytes, or granulocytes.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Armenian Hamster
Immunogen: Mouse N-terminus of CXCR3
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested Block - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone CXCR3-173 does not work for Western blotting.7 For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 126526) with endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered.
Application References(PubMed link indicates BioLegend citation): Krug A, et al. 2002. J. Immunol. 169:6079. McAleer JP, et al. 2009. J. Immunol. 182:5322. PubMed Sharma R. 2009. J. Immunol. 183:3212. (FC) PubMed Barbi J, et al. 2009. FASEB J. 23:3990. PubMed Kimpfler S, et al. 2009. J. Immunol. 183:6330. PubMed Dürr C, et al. 2010. Cancer Res. 70:10170. (Block) PubMed Uppaluri R, et al. 2008. Transplantation 86:137.
Product Citations: Haworth O, et al. 2011. J Immunol. 186:6129. PubMed Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed Yu H, et al. 2020. Front Immunol. 11:1773. PubMed Hailemichael Y, et al. 2022. Cancer Cell. 40:509. PubMed Uzhachenko RV, et al. 2021. Cell Reports. 35(1):108944. PubMed Wei SC et al. 2017. Cell. 170(6):1120-1133 . PubMed Wei SC, et al. 2019. Immunity. 50:1084. PubMed Joseph R, et al. 2021. Br J Cancer. 125:176. PubMed Martin F, et al. 2011. Infect Immun. 79:1898. PubMed Dürr C, et al. 2010. Cancer Res. 70:10170. PubMed Pein M, et al. 2020. Nat Commun. 11:1494. PubMed Dionisio-Santos DA, et al. 2021. Front Neurosci. 15:758677. PubMed Zhu YP et al. 2018. Cell reports. 24(9):2329-2341 . PubMed Seung E, et al. 2011. J Immunol. 186:6830. PubMed
RRID: AB_1027635 (BioLegend Cat. No. 126502)
Distribution: T cells, plasmacytoid dendritic cells, GM-CSF activated CD34+ hematopoietic progenitors
Function: Essential in T cell recruitment to sites of inflammation
Cell Type: Dendritic cells, Hematopoietic stem and progenitors, T cells
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors, GPCR
Gene ID: 12766
UniProt: View information about CD183 on UniProt.org
Clone: CXCR3-173
Regulatory Status: RUO
Other Names: CXCR3, G protein-coupled receptor 9 (GPR9), CKR-L2, IP10 receptor (IP10-R), Mig receptor (Mig-R )
Isotype: Armenian Hamster IgG