Biolegend, 126515, PE/Cyanine7 anti-mouse CD183 (CXCR3) Antibody, 25μg

CD183, also known as CXCR3, is a member of the C-X-C chemokine family, characterized by a pair of cysteine residues separated by a single amino acid. CXCR3 is a 38 kD seven pass transmembrane receptor coupled to G-protein. It mediates Ca 2+ mobilization and chemotaxis in response to C-X-C chemokines, such as IP10 (CXCL10), MIG (CXCL9), I-TAC (CXCL11) and PF4 (CXCL4). CXCR3 is expressed primarily on activiated T lymphocytes, NK cells, and some epithelial cells and endothelial cells. It is not expressed on B cells, monocytes, or granulocytes.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Armenian Hamster
Immunogen: Mouse N-terminus of CXCR3
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Clone CXCR3-173 does not work for Western blotting.7 For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 126526) with endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered.
Application References(PubMed link indicates BioLegend citation): Krug A, et al. 2002. J. Immunol. 169:6079. McAleer JP, et al. 2009. J. Immunol. 182:5322. PubMed Sharma R. 2009. J. Immunol. 183:3212. (FC) PubMed Barbi J, et al. 2009. FASEB J. 23:3990. PubMed Kimpfler S, et al. 2009. J. Immunol. 183:6330. PubMed Dürr C, et al. 2010. Cancer Res. 70:10170. (Block) PubMed Uppaluri R, et al. 2008. Transplantation 86:137.
Product Citations: Prota G, et al. 2015. Vaccine. 410: 01290-6. PubMed Christian DA, et al. 2022. Sci Immunol. 7:eabq7432. PubMed Wang D, et al. 2018. Immunity. 48:659. PubMed Amir M, et al. 2018. Cell Rep. 25:3733. PubMed Chen L, et al. 2020. J Exp Med. 217:00:00. PubMed He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed Alves C, et al. 2015. PLoS One. 10: 0142972. PubMed Dietmar Herndler‐Brandstetter et al. 2018. Immunity. 48(4):716-729 . PubMed Nakajima T, et al. 2021. J Exp Med. 218:. PubMed Evgin L, et al. 2020. Nat Commun. 2.671527778. PubMed Phalke SP, et al. 2019. PLoS One. 14:e0218827. PubMed Brenndörfer E, et al. 2014. J Immunol. 192:1671. PubMed Forero A, et al. 2020. Immunity. 51(3):451-464. PubMed Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed Gaya M et al. 2018. Cell. 172(3):517-533 . PubMed
RRID: AB_2086740 (BioLegend Cat. No. 126515) AB_2245493 (BioLegend Cat. No. 126516)
Distribution: T cells, plasmacytoid dendritic cells, GM-CSF activated CD34+ hematopoietic progenitors
Function: Essential in T cell recruitment to sites of inflammation
Cell Type: Dendritic cells, Hematopoietic stem and progenitors, T cells
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors, GPCR
Gene ID: 12766
UniProt: View information about CD183 on UniProt.org
Clone: CXCR3-173
Regulatory Status: RUO
Other Names: CXCR3, G protein-coupled receptor 9 (GPR9), CKR-L2, IP10 receptor (IP10-R), Mig receptor (Mig-R )
Isotype: Armenian Hamster IgG