Biolegend, 126605, FITC anti-mouse CD8b Antibody, 50μg

CD8b is the 32 kD β chain of CD8, also known as Lyt-3 or Ly-3. It is a member of the Ig superfamily expressed as a heterodimer with the CD8α chain on a subset of MHC class I-restricted T cells and most thymocytes. CD8 is a co-receptor for the TCR complex involved in T cell activation.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse thymocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen tissue sections.
Application References(PubMed link indicates BioLegend citation): McNical AM, et al. 2007. Eur. J. Immunol. 37:1634.
Product Citations: Christian DA, et al. 2022. Sci Immunol. 7:eabq7432. PubMed Wang YC, et al. 2023. J Exp Med. 220:. PubMed Xiong T, et al. 2022. Cancer Sci. 113:1955. PubMed Dean JW 2023. Cell Reports. 42(1):111963. PubMed Drijvers JM, et al. 2021. Cancer Immunol Res. 9:184. PubMed Misumi I et al. 2019. Cell Rep. 27(5):1387-1396 . PubMed Misumi I et al. 2019. Cell Rep. 27(2):514-524 . PubMed LaFleur MW, et al. 2019. Nat Immunol. 20:1335. PubMed Mardomi A, et al. 2021. J Cell Physiol. 236:6328. PubMed Canto F, et al. 2016. Sci Rep. 6:28573. PubMed Bianchi JJ, et al. 2019. Cell Rep. 27:2847. PubMed Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed Moon S, et al. 2021. J Exp Med. 218:. PubMed Nelson CE et al. 2019. Cell Rep. 28(12):3092-3104 . PubMed Takaku S, et al. 2022. Evid Based Complement Alternat Med. 2022:6122955. PubMed Lee-Chang C, et al. 2021. J Exp Med. 218:. PubMed Nakagawa Y, et al. 2012. Int Immunol. 25:11. PubMed Wells AC et al. 2017. eLife. 6 pii: e26398. PubMed Viaud M, et al. 2020. Cell Reports. 30(10):3397-3410. PubMed Guo Y, et al. 2021. Nat Immunol. 22:746. PubMed
RRID: AB_961293 (BioLegend Cat. No. 126605) AB_961295 (BioLegend Cat. No. 126606)
Structure: Ig superfamily, CD8β chain, 34 kD
Distribution: Majority of thymocytes, cytotoxic T cell subsets
Function: Co-receptor of TCR
Ligand/Receptor: MHC class I
Cell Type: T cells, Thymocytes, Tregs
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1.Barclay A, et al. 1997. The Leukocyte antigen Facts Book Academic Press. 2. Zamoyska R. 1994. Immunity 1:243-246. 3. Ellmeier W, et al. 1999. Annu. Rev Immunol 17:523. 4. Ledbetter JA, et al. 1981. J. Exp. Med. 153:1503.
Gene ID: 12526
UniProt: View information about CD8beta on UniProt.org
Clone: YTS156.7.7
Regulatory Status: RUO
Other Names: Lyt-3, Ly-3
Isotype: Rat IgG2b, κ