Biolegend, 126609, PerCP/Cyanine5.5 anti-mouse CD8b Antibody, 25μg

CD8b is the 32 kD β chain of CD8, also known as Lyt-3 or Ly-3. It is a member of the Ig superfamily expressed as a heterodimer with the CD8α chain on a subset of MHC class I-restricted T cells and most thymocytes. CD8 is a co-receptor for the TCR complex involved in T cell activation.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse thymocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen tissue sections.
Application References(PubMed link indicates BioLegend citation): McNical AM, et al. 2007. Eur. J. Immunol. 37:1634.
Product Citations: Lucca L, et al. 2014. J Immunol. 193:3267. PubMed Konjar S, et al. 2022. Proc Natl Acad Sci U S A. 119:e2202144119. PubMed Mackin S, et al. 2023. Nat Microbiol. 8:569. PubMed Lin YH 2023. Immunity. 56(1):207-223.e8. PubMed Fiege JK, et al. 2021. Cell Host Microbe. 29:1815. PubMed Zhang W, et al. 2022. Dev Cell. 57:329. PubMed Marco Barros R, et al. 2016. Cell. 167: 203-218. PubMed Acker A, et al. 2014. PLoS One. 9:110015. PubMed Gimblet C et al. 2017. Cell host & microbe. 22(1):13-24 . PubMed Zhu B, et al. 2021. Immunity. 54(6):1200-1218.e9. PubMed LaFleur MW, et al. 2019. Nat Immunol. 20:1335. PubMed Jin RM, et al. 2017. J Immunol. 198:352. PubMed Hossain DMS, et al. 2018. J Clin Invest. 128:644. PubMed Hum NR, et al. 2022. Front Oncol. 12:928474. PubMed Panea C, et al. 2021. Commun Biol. 4:913. PubMed de Reuver R, et al. 2021. Cell Reports. 36(6):109500. PubMed LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed Hassan AO, et al. 2020. Cell. 183:169. PubMed Jandke A, et al. 2020. Nat Commun. 3.075694444. PubMed Gubin MM, et al. 2018. Cell. 175:1014. PubMed Raju S, et al. 2020. Cell Reports. 29(9):2556-2564.e3.. PubMed Salazar V, et al. 2019. Cell Rep. 26:1585. PubMed Bennion BG, et al. 2019. J Virol. 93. PubMed Chao JL, et al. 2021. Cell Rep Med. 2:100399. PubMed Sivaram N, et al. 2019. J Clin Invest. 130. PubMed Kerdidani D, et al. 2022. J Exp Med. 219:. PubMed Wang Y, et al. 2022. Nat Cell Biol. 24:230. PubMed
RRID: AB_961304 (BioLegend Cat. No. 126609) AB_2260149 (BioLegend Cat. No. 126610)
Structure: Ig superfamily, CD8β chain, 34 kD
Distribution: Majority of thymocytes, cytotoxic T cell subsets
Function: Co-receptor of TCR
Ligand/Receptor: MHC class I
Cell Type: T cells, Thymocytes, Tregs
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1.Barclay A, et al. 1997. The Leukocyte antigen Facts Book Academic Press. 2. Zamoyska R. 1994. Immunity 1:243-246. 3. Ellmeier W, et al. 1999. Annu. Rev Immunol 17:523. 4. Ledbetter JA, et al. 1981. J. Exp. Med. 153:1503.
Gene ID: 12526
UniProt: View information about CD8beta on UniProt.org
Clone: YTS156.7.7
Regulatory Status: RUO
Other Names: Lyt-3, Ly-3
Isotype: Rat IgG2b, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.