Biolegend, 127201, Purified anti-mouse CD73 Antibody, 25μg

CD73 (ecto-5'-nucleotidase) is a 69 kD GPI-anchored surface protein. In mice, expression of CD73 in bone marrow is restricted to CD11b + myeloid cells. In spleen, it is largely expressed on T cells.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: CHO cells transfected with mouse CD73
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested IHC-F - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 5.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Yamshita Y, et al 1998. Eur. J. Immunol. 28:2981. Bentley JK, et al. 2010. Respir. Res. 11:127. (IHC-P)
Product Citations: Rafaiee R, et al. 2023. Bioimpacts. 13:97. PubMed Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed Campisi L, et al. 2022. Nature. 606:945. PubMed Dienz O, et al. 2020. J Immunol. 204:1521. PubMed Xie D, et al. 2020. Eur J Immunol. 50:1729. PubMed Prideaux M, et al. 2021. Sci Rep. 11:22593. PubMed
RRID: AB_1089066 (BioLegend Cat. No. 127202)
Structure: 69 kD GPI-anchored surface protein
Distribution: Positive on most CD11b+ myeloid cells in bone marrow, but not on CD11b+ peripheral cells; a large proportion of CD4+ and CD8+ splenic T cells express CD73
Function: A nucleotidase catalyzing the dephosphorylation of extracellular nucleoside 5' monophosphates
Ligand/Receptor: Nucleoside 5' monophosphate
Cell Type: B cells, Mesenchymal Stem Cells, Tregs
Biology Area: Costimulatory Molecules, Immunology, Stem Cells
Molecular Family: CD Molecules
Antigen References: 1. Yamashita Y, et al 1998. Eur. J. Immunol. 28:2981. 2. Resta R, et al. 1998. Immunol. Rev. 161:95.
Regulation: Expression is regulated by the development and maturation of leukocytes
Gene ID: 23959
UniProt: View information about CD73 on UniProt.org
Clone: TY/11.8
Regulatory Status: RUO
Other Names: Ecto-5'-nucleotidase
Isotype: Rat IgG1, κ