Biolegend, 127205, PE anti-mouse CD73 Antibody, 25μg

CD73 (ecto-5'-nucleotidase) is a 69 kD GPI-anchored surface protein. In mice, expression of CD73 in bone marrow is restricted to CD11b + myeloid cells. In spleen, it is largely expressed on T cells.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: CHO cells transfected with mouse CD73
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): Yamshita Y, et al 1998. Eur. J. Immunol. 28:2981. Bentley JK, et al. 2010. Respir. Res. 11:127. (IHC-P)
Product Citations: Li L, et al. 2017. FASEB J. 31:1067. PubMed Schwarz A, et al. 2023. Front Immunol. 14:1038689. PubMed Giampaolo S, et al. 2023. iScience. 26:106234. PubMed Vasanthakumar A, et al. 2020. Nature. 579:581. PubMed Fan C, et al. 2020. Stem Cell Res Ther. 0.765277778. PubMed Chen ELY, et al. 2021. Cell Rep. 35:109227. PubMed Xu L et al. 2017. Immunity. 47(3):538-551 . PubMed Sutherland TE, et al. 2021. Frontiers in Immunology. 12:715209. PubMed Ortín-Martínez A, et al. 2021. EMBO J. 40:e107264. PubMed Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed Gabriel SS, et al. 2021. Immunity. 54(8):1698-1714.e5. PubMed Santini MP, et al. 2020. Cell Rep. 30:555. PubMed Soleimani A, et al. 2022. Sci Rep. 12:10423. PubMed Xu C, et al. 2021. Cell Reports. 35(11):109235. PubMed Zilionis R et al. 2019. Immunity. 50(5):1317-1334 . PubMed Timilshina M, et al. 2020. Cell Reports. 27(10):2948-2961.e7.. PubMed
RRID: AB_1089065 (BioLegend Cat. No. 127205) AB_2154094 (BioLegend Cat. No. 127206)
Structure: 69 kD GPI-anchored surface protein
Distribution: Positive on most CD11b+ myeloid cells in bone marrow, but not on CD11b+ peripheral cells; a large proportion of CD4+ and CD8+ splenic T cells express CD73
Function: A nucleotidase catalyzing the dephosphorylation of extracellular nucleoside 5' monophosphates
Ligand/Receptor: Nucleoside 5' monophosphate
Cell Type: B cells, Mesenchymal Stem Cells, Tregs
Biology Area: Costimulatory Molecules, Immunology, Stem Cells
Molecular Family: CD Molecules
Antigen References: 1. Yamashita Y, et al 1998. Eur. J. Immunol. 28:2981. 2. Resta R, et al. 1998. Immunol. Rev. 161:95.
Regulation: Expression is regulated by the development and maturation of leukocytes
Gene ID: 23959
UniProt: View information about CD73 on UniProt.org
Clone: TY/11.8
Regulatory Status: RUO
Other Names: Ecto-5'-nucleotidase
Isotype: Rat IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.