Biolegend, 127213, PerCP/Cyanine5.5 anti-mouse CD73 Antibody, 25μg

CD73 (ecto-5'-nucleotidase) is a 69 kD GPI-anchored surface protein. In mice, expression of CD73 in bone marrow is restricted to CD11b + myeloid cells. In spleen, it is largely expressed on T cells.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: CHO cells transfected with mouse CD73
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): Yamshita Y, et al 1998. Eur. J. Immunol. 28:2981. Bentley JK, et al. 2010. Respir. Res. 11:127. (IHC-P)
Product Citations: Ogishi M, et al. 2023. J Exp Med. 220: . PubMed Hirata Y et al. 2018. Cell stem cell. 22(3):445-453 . PubMed Dumas SJ, et al. 2021. STAR Protocols. 2(3):100523. PubMed Rodda LB et al. 2018. Immunity. 48(5):1014-1028 . PubMed Chen ELY, et al. 2021. Cell Rep. 35:109227. PubMed Duan L, et al. 2021. Immunity. 54:2256. PubMed Mintz MA, et al. 2019. Immunity. 51:310. PubMed Dumas SJ, et al. 2020. J Am Soc Nephrol. 31:118. PubMed Wei C, et al. 2016. Cell Death Dis. 7:e2489. PubMed Zhou J, et al. 2019. Nat Commun. 10:2427. PubMed Raso F, et al. 2018. J Clin Invest. 128:4163. PubMed Young JS, et al. 2018. Front Immunol. 9:1385. PubMed Ott M, et al. 2020. JCI Insight. 5(17):134386. PubMed
RRID: AB_11219608 (BioLegend Cat. No. 127213) AB_11219403 (BioLegend Cat. No. 127214)
Structure: 69 kD GPI-anchored surface protein
Distribution: Positive on most CD11b+ myeloid cells in bone marrow, but not on CD11b+ peripheral cells; a large proportion of CD4+ and CD8+ splenic T cells express CD73
Function: A nucleotidase catalyzing the dephosphorylation of extracellular nucleoside 5' monophosphates
Ligand/Receptor: Nucleoside 5' monophosphate
Cell Type: B cells, Mesenchymal Stem Cells, Tregs
Biology Area: Costimulatory Molecules, Immunology, Stem Cells
Molecular Family: CD Molecules
Antigen References: 1. Yamashita Y, et al 1998. Eur. J. Immunol. 28:2981. 2. Resta R, et al. 1998. Immunol. Rev. 161:95.
Regulation: Expression is regulated by the development and maturation of leukocytes
Gene ID: 23959
UniProt: View information about CD73 on UniProt.org
Clone: TY/11.8
Regulatory Status: RUO
Other Names: Ecto-5'-nucleotidase
Isotype: Rat IgG1, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.