Biolegend, 127216, Brilliant Violet 605™ anti-mouse CD73 Antibody, 50μg

CD73 (ecto-5'-nucleotidase) is a 69 kD GPI-anchored surface protein. In mice, expression of CD73 in bone marrow is restricted to CD11b + myeloid cells. In spleen, it is largely expressed on T cells.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: CHO cells transfected with mouse CD73
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application References(PubMed link indicates BioLegend citation): Yamshita Y, et al 1998. Eur. J. Immunol. 28:2981. Bentley JK, et al. 2010. Respir. Res. 11:127. (IHC-P)
Product Citations: Hirata Y et al. 2018. Cell stem cell. 22(3):445-453 . PubMed Andrade MS, et al. 2022. JCI Insight. 7:. PubMed Hu W, et al. 2021. Nat Immunol. 22:1163. PubMed Waide ML, et al. 2020. Cell Rep. 33:108503. PubMed Perrot I et al. 2019. Cell Rep. 27(8):2411-2425 . PubMed Suah AN, et al. 2021. J Clin Invest. 131:. PubMed Dikiy S, et al. 2021. Immunity. 54(5):931-946.e11. PubMed Campbell C et al. 2018. Immunity. 48(6):1245-1257 . PubMed Abbott RK, et al. 2018. Immunity. 48:133. PubMed Quiroz HJ, et al. 2022. Front Cardiovasc Med. 8:826687. PubMed Watson MJ, et al. 2021. Nature. 591:645. PubMed Singhal P, et al. 2016. Proc Natl Acad Sci U S A. 113: 122 - 127. PubMed Jones D, et al. 2015. J Immunol. 195: 4753 - 4759. PubMed Shen H, et al. 2022. Nat Commun. 13:5013. PubMed
RRID: AB_2561528 (BioLegend Cat. No. 127215)
Structure: 69 kD GPI-anchored surface protein
Distribution: Positive on most CD11b+ myeloid cells in bone marrow, but not on CD11b+ peripheral cells; a large proportion of CD4+ and CD8+ splenic T cells express CD73
Function: A nucleotidase catalyzing the dephosphorylation of extracellular nucleoside 5' monophosphates
Ligand/Receptor: Nucleoside 5' monophosphate
Cell Type: B cells, Mesenchymal Stem Cells, Tregs
Biology Area: Costimulatory Molecules, Immunology, Stem Cells
Molecular Family: CD Molecules
Antigen References: 1. Yamashita Y, et al 1998. Eur. J. Immunol. 28:2981. 2. Resta R, et al. 1998. Immunol. Rev. 161:95.
Regulation: Expression is regulated by the development and maturation of leukocytes
Gene ID: 23959
UniProt: View information about CD73 on UniProt.org
Clone: TY/11.8
Regulatory Status: RUO
Other Names: Ecto-5'-nucleotidase
Isotype: Rat IgG1, κ