Biolegend, 127217, Brilliant Violet 421™ anti-mouse CD73 Antibody, 50μg

CD73 (ecto-5'-nucleotidase) is a 69 kD GPI-anchored surface protein. In mice, expression of CD73 in bone marrow is restricted to CD11b + myeloid cells. In spleen, it is largely expressed on T cells.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: CHO cells transfected with mouse CD73
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested IHC-F - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 5.0 - 10.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application References(PubMed link indicates BioLegend citation): Yamshita Y, et al 1998. Eur. J. Immunol. 28:2981. Bentley JK, et al. 2010. Respir. Res. 11:127. (IHC-P)
Product Citations: Schädlich IS, et al. 2022. iScience. 25:104470. PubMed Vesin B, et al. 2022. Mol Ther. 30:2984. PubMed Emmerson A, et al. 2018. J Clin Invest. 128:3088. PubMed Grauel AL, et al. 2020. Nat Commun. 4.84375. PubMed
RRID: AB_2687251 (BioLegend Cat. No. 127217)
Structure: 69 kD GPI-anchored surface protein
Distribution: Positive on most CD11b+ myeloid cells in bone marrow, but not on CD11b+ peripheral cells; a large proportion of CD4+ and CD8+ splenic T cells express CD73
Function: A nucleotidase catalyzing the dephosphorylation of extracellular nucleoside 5' monophosphates
Ligand/Receptor: Nucleoside 5' monophosphate
Cell Type: B cells, Mesenchymal Stem Cells, Tregs
Biology Area: Costimulatory Molecules, Immunology, Stem Cells
Molecular Family: CD Molecules
Antigen References: 1. Yamashita Y, et al 1998. Eur. J. Immunol. 28:2981. 2. Resta R, et al. 1998. Immunol. Rev. 161:95.
Regulation: Expression is regulated by the development and maturation of leukocytes
Gene ID: 23959
UniProt: View information about CD73 on UniProt.org
Clone: TY/11.8
Regulatory Status: RUO
Other Names: Ecto-5'-nucleotidase
Isotype: Rat IgG1, κ
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.