Biolegend, 127409, APC anti-mouse Podoplanin Antibody, 25μg

The mucin-type glycoprotein podoplanin is thought to be involved in the development of the lymphatic vascular system. Podoplanin is named after its expression in the kidney glomerular epithelial cells (podocytes). It has a potential role in tumor progression.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Syrian Hamster
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemistry6, and spatial biology (IBEX)8,9.
Application References(PubMed link indicates BioLegend citation): Farr A, et al. 1992. J. Histochem. Cytochem. 40:651. Farr AG, et al. 1992. J. Exp. Med. 176:1477. Bekiaris V, et al. 2008. J. Immunol. 180:6768. Algars A, et al. 2011. Blood 117:4387. PubMed Reis VO, et al. 2012. Immunobiology. 217:831. PubMed Kaji C, et al. 2012. Acta. Histochem. Cytochem. 45:227. (IHC) Kretschmer S, et al. 2013. PLoS One. 8:e52201. PubMed. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Cheong LY, et al. 2023. Nat Commun. 14:1213. PubMed Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed Buechler MB, et al. 2021. Nature. 593:575. PubMed Ozga AJ, et al. 2022. Immunity. 55:82. PubMed Garnier L, et al. 2022. Sci Adv. 8:eabl5162. PubMed Jiang W, et al. 2022. Redox Biol. 56:102432. PubMed Krishnamurty AT, et al. 2022. Nature. 611:148. PubMed Tomiaki C, et al. 2022. Front Immunol. 13:1014462. PubMed Sastourné-Arrey Q, et al. 2023. Nat Commun. 14:80. PubMed Urban SK, et al. 2020. Liver Int. 40:3103. PubMed Weeden C, et al. 2017. PLoS Biol. 10.1371/journal.pbio.2000731. PubMed McCarthy N, et al. 2020. Cell Stem Cell. 391:26. PubMed Rodda LB et al. 2018. Immunity. 48(5):1014-1028 . PubMed Seidenberg J, et al. 2021. Int J Mol Sci. :22. PubMed Brinkman C, et al. 2013. J Immunol. 191:2412. PubMed Schwab M, et al. 2021. Exp Dermatol. 30:1619. PubMed Davidson S, et al. 2020. Cell Reports. 31(7):107628. PubMed Jackson-Jones LH, et al. 2020. Immunity. 52:700. PubMed Duan L, et al. 2021. Immunity. 54:2256. PubMed Donati Y, et al. 2020. Am J Physiol Lung Cell Mol Physiol. L619:318. PubMed Walsh SM, et al. 2021. eLife. 10:00. PubMed Grauel AL, et al. 2020. Nat Commun. 4.84375. PubMed Clement CC, et al. 2021. Nat Commun. 12:4447. PubMed Matthew B Buechler et al. 2019. Immunity. 51(1):119-130 . PubMed Denton A, et al. 2014. Proc Natl Acad Sci U S A. 111:12139. PubMed Bellomo A, et al. 2020. Immunity. 53(1):127-142.e7. PubMed Eisemann T, et al. 2019. Front Oncol. 9:187. PubMed Shaikh H, et al. 2021. Front Immunol. 12:689896. PubMed Singhal P, et al. 2016. Proc Natl Acad Sci U S A. 113: 122 - 127. PubMed Blaskovic S, et al. 2020. Am J Physiol Lung Cell Mol Physiol. L606:318. PubMed Coulombe P et al. 2019. Cell reports. 27(6):1769-1780 . PubMed Hara T, et al. 2021. Cancer Cell. 39(6):779-792.e11. PubMed Hirota K et al. 2018. Immunity. 48(6):1220-1232 . PubMed Kurashima Y, et al. 2021. Nat Commun. 12:1067. PubMed Tadayon S, et al. 2021. Front Immunol. 12:602122. PubMed Costa B, et al. 2019. Blood Adv. 3:1092. PubMed
RRID: AB_10612940 (BioLegend Cat. No. 127409) AB_10613649 (BioLegend Cat. No. 127410)
Structure: 43 Kd glycosylated type-1 transmembrane protein. Mucin-type protein.
Distribution: Expressed on epithelial and mesothelial cells.
Cell Type: Epithelial cells
Biology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Antigen References: 1. Farr A, et al. 1992. J. Histochem. Cytochem. 40:651. 2. Schacht V, et al. 2005. Am. J. Pathol. 166:913.
Gene ID: 14726
UniProt: View information about Podoplanin on UniProt.org
Clone: 8.1.1
Regulatory Status: RUO
Other Names: T1a, gp38, stromal cell marker
Isotype: Syrian Hamster IgG