Biolegend, 127423, Brilliant Violet 421™ anti-mouse Podoplanin Antibody, 50μg

The mucin-type glycoprotein podoplanin is thought to be involved in the development of the lymphatic vascular system. Podoplanin is named after its expression in the kidney glomerular epithelial cells (podocytes). It has a potential role in tumor progression.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Syrian Hamster
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemistry6, and spatial biology (IBEX)8,9.
Application References(PubMed link indicates BioLegend citation): Farr A, et al. 1992. J. Histochem. Cytochem. 40:651. Farr AG, et al. 1992. J. Exp. Med. 176:1477. Bekiaris V, et al. 2008. J. Immunol. 180:6768. Algars A, et al. 2011. Blood 117:4387. PubMed Reis VO, et al. 2012. Immunobiology. 217:831. PubMed Kaji C, et al. 2012. Acta. Histochem. Cytochem. 45:227. (IHC) Kretschmer S, et al. 2013. PLoS One. 8:e52201. PubMed. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Lucas B, et al. 2023. Nat Commun. 14:2066. PubMed Ali M, et al. 2022. JCI Insight. 7:. PubMed Kolodkin-Gal D, et al. 2021. Gut. Online ahead of print. PubMed Shaashua L, et al. 2022. Nat Commun. 13:6513. PubMed
RRID: AB_2814017 (BioLegend Cat. No. 127423)
Structure: 43 Kd glycosylated type-1 transmembrane protein. Mucin-type protein.
Distribution: Expressed on epithelial and mesothelial cells.
Cell Type: Epithelial cells
Biology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Antigen References: 1. Farr A, et al. 1992. J. Histochem. Cytochem. 40:651. 2. Schacht V, et al. 2005. Am. J. Pathol. 166:913.
Gene ID: 14726
UniProt: View information about Podoplanin on UniProt.org
Clone: 8.1.1
Regulatory Status: RUO
Other Names: T1a, gp38, stromal cell marker
Isotype: Syrian Hamster IgG
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.