Biolegend, 127606, FITC anti-mouse Ly-6G Antibody, 500μg

Lymphocyte antigen 6 complex, locus G (Ly-6G), a 21-25 kD GPI-anchored protein, is expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes.
500μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Ly-6G transfected EL-4J cell line.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for other applications.
Excitation Laser: Blue Laser (488 nm)
Application Notes: While 1A8 recognizes only Ly-6G, clone RB6-8C5 recognizes both Ly-6G and Ly-6C. Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C15. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A815. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.416. Additional reported applications (for the relevant formats) include: immunohistochemistry9 of frozen sections10 and paraffin-embedded sections11, depletion4, 12-14, and spatial biology (IBEX)20,21. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for in vivo studies or highly sensitive assays (Cat. No. 127632, 127649, 127650, 127661 and 127662).
Application References(PubMed link indicates BioLegend citation): Fleming TJ, et al. 1993. J. Immunol. 151:2399. (FC) Daley JM, et al. 2008. J. Leukocyte Biol. 83:1. (FC) Dietlin TA, et al. 2007. J. Leukocyte Biol. 81:1205. (FC) Daley J, et al. 2007. J. Leukocyte Biol. doi:10.1189. (Deplete) PubMed Tadagavadi RK, et al. 2010. J. Immunol. 185:4904. PubMed Sumagin R, et al. 2010. J. Immunol. 185:7057. PubMed Guiducci C, et al. 2010. J. Exp Med. 207:2931. PubMed Fujita M, et al. 2011. Cancer Res. 71:2664. PubMed Van Leeuwen, et al. 2008. Arterioscler. Thromb. Vasc. Biol. 28:84. (IHC) Kowanetz M, et al. 2010. P. Natl. Acad. Sci. USA 107:21248. [supplementary data] (IHC) Esbona K, et al. 2016. Breast Cancer Res. 18:35. (IHC) Wojtasiak M, et al. 2010. J. Gen. Virol. 91:2158. (FC, Deplete) Jaeger BN, et al. 2012. J. Exp. Med. 209:565. (Deplete) Wozniak KL, et al. 2012. BMC Immunol. 13:65 (FC, Deplete) Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538. Ng LG, et al. 2011. J Invest. Dermatol. 131:2058. PubMed Ma C, et al. 2012. J. Leukoc. Biol. 92:1199. McCartney-Francis, N, et al. 2014. J Leukoc. Biol. 96:917. PubMed Her Z, et al. 2014. EMBO Mol. Med. 7:24. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Valladao A, et al. 2016. J Immunol. 197(12):4541-4551. PubMed Campbell IK, Leong D 2016. J Immunol. 197(11):4392-4402. PubMed Sun L, et al. 2021. Cancer Cell. 39:1361. PubMed Frieler RA, et al. 2017. Exp Neurol. 298:104. PubMed Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed Tomida S, et al. 2019. Sci Rep. 9:10751. PubMed Gordan S, et al. 2020. Cell Reports. 29(10):3033-3046.e4.. PubMed Sanmarco LM, et al. 2022. Nature. 611:801. PubMed Peng J, et al. 2023. Am J Reprod Immunol. 89:e13678. PubMed Le DT, et al. 2023. iScience. 26:106059. PubMed Veras FP, et al. 2023. Respir Res. 24:66. PubMed Doke T, et al. 2023. Nat Metab. 5:414. PubMed Tang C, et al. 2023. Nat Commun. 14:1493. PubMed Senatus L, et al. 2023. Commun Biol. 6:280. PubMed Ma X, et al. 2023. Nat Commun. 14:1606. PubMed Park JA, et al. 2023. J Immunother Cancer. 11:. PubMed Chung JY, et al. 2023. Nat Commun. 14:1794. PubMed Zhu CL, et al. 2023. Int J Biol Sci. 19:1413. PubMed Silva BM, et al. 2023. J Clin Invest. 133:. PubMed Cui B, et al. 2023. Int Immunopharmacol. 120:110240. PubMed Oh J, et al. 2023. Nat Commun. 14:3278. PubMed Pohlmeier L, et al. 2021. Allergy. 76:2030. PubMed Li T, et al. 2022. J Exp Med. 219: . PubMed Yang Y, et al. 2022. FEBS J. 289:5279. PubMed Yue Y, et al. 2022. Nat Biomed Eng. 6:898. PubMed Zhu Y, et al. 2023. J Ethnopharmacol. 301:115763. PubMed Xu Y, et al. 2023. EMBO Rep. 24:e55503. PubMed Xu H, et al. 2022. Aging Dis. 13:1875. PubMed Pavliuchenko N, et al. 2023. Front Immunol. 13:1035226. PubMed Tsioti I, et al. 2023. J Neuroinflammation. 20:25. PubMed Zong P, et al. 2022. Nat Cardiovasc Res. 1:344. PubMed Kim S, et al. 2020. Immunity. 53(4):759-774.e9. PubMed Garcia-Fabiani MB, et al. 2020. Methods Enzymol. 632:369. PubMed Miller CM, et al. 2020. J Virol. 94:00:00. PubMed Long M, et al. 2017. J Immunol. 198(2):862-872. PubMed Oliveira-Lima OC, et al. 2015. Immunobiology. . PubMed Mandraju R, et al. 2014. J Immunol. 192:4303. PubMed Golden GJ, et al. 2021. MBio. 12:e0118121. PubMed He R, et al. 2021. J Immunol. 206:2353. PubMed Lefebvre MN, et al. 2021. Cell Rep. 37:109956. PubMed Suresh R, et al. 2020. J Immunother Cancer. 8:. PubMed Okajima T, et al. 2021. Front Immunol. 12:738041. PubMed Tripathi H, et al. 2020. J Mol Cell Cardiol. 149:95. PubMed Clark K, et al. 2015. J Autoimmun. Available online 21 August 2015. PubMed Mogilenko DA, et al. 2020. Immunity. 54(1):99-115.e12. PubMed Patsalos A, et al. 2019. J Immunol. 203:1532. PubMed Lin R, et al. 2020. Sci Rep. 10:14397. PubMed Song J, et al. 2021. J Am Heart Assoc. 10:e017329. PubMed Yang C, et al. 2021. J Immunother Cancer. 9:. PubMed Jiang W, et al. 2021. Oncol Lett. 22:625. PubMed Johnson KD, et al. 2022. Blood Adv. 6:1464. PubMed Jenull S, et al. 2021. Cell Reports. 36(3):109406. PubMed Torre S, et al. 2016. Nat Immunol. 10.1038/ni.3581. PubMed Montufar-Solis D, Klein J 2016. PLoS One. 11: 014611. PubMed Wang C, et al. 2015. Sci Rep. 5: 14124. PubMed Huai W, et al. 2019. J Exp Med. 216:772. PubMed Han J, et al. 2022. FEBS J. 289:417. PubMed Larsen SE, et al. 2021. Sci Rep. 11:9040. PubMed Castiglioni A, et al. 2015. PLoS One. 10:128094. PubMed Guesdon W, et al. 2015. J Infect Dis. 212: 1332 - 1340. PubMed Strother R, et al. 2016. J Immunol. 197(11):4301-4311. PubMed Rao S, et al. 2017. Cell. 168(3):503-516.e12. PubMed LeBlond ND, et al. 2020. J Lipid Res. 61:1697. PubMed Park JA, et al. 2021. J Hematol Oncol. 14:142. PubMed Bae SH, et al. 2021. Theranostics. 11:2522. PubMed Enriquez AB, et al. 2022. iScience. 25:104305. PubMed Mitsune A, et al. 2021. Respir Res. 22:232. PubMed Dieterich LC, et al. 2019. International Journal of Cancer. 145(10):2804-2815. PubMed Diao N, et al. 2016. Sci Rep. 6:34672. PubMed Ellis G, et al. 2015. EMBO Rep. 16: 1203-1218. PubMed Fujikura D, et al. 2013. PLoS One. 8:e55321. PubMed Ueki H, et al. 2020. Nat Protoc. 15:1041. PubMed Saika A, et al. 2020. FASEB Bioadv. 2:59. PubMed Nguyen CM, et al. 2019. Diabetes. 68:1499. PubMed Wang H et al. 2017. Developmental cell. 40(6):566-582 . PubMed Pan W et al. 2017. Immunity. 47(2):284-297 . PubMed Billi AC, et al. 2019. JCI Insight. 4:8. PubMed Han YH, et al. 2021. Science. 373:. PubMed Abad C, et al. 2014. J Leukoc Biol. 95:357. PubMed Syed A, et al. 2015. Infect Immun . 83: 3428-3437. PubMed Liu Z, et al. 2016. J Biol Chem. 291: 1441 - 1447. PubMed Ratitong B, et al. 2021. Cell Reports. 35(7):109139. PubMed Na YR, et al. 2020. Cell Rep. 107643:31. PubMed Wang L, et al. 2017. Front Immunol.. 10.3389/fimmu.2017.01754. PubMed Luan HH et al. 2019. Cell. 178(5):1231-1244 . PubMed Cheng K, et al. 2021. Nat Commun. 12:2041. PubMed Enriquez AB, et al. 2022. iScience. 25:104305. PubMed Wu X, et al. 2021. Elife. 10:. PubMed Opzoomer JW, et al. 2021. Sci Adv. 7:eabg9518. PubMed Aegerter H, et al. 2020. Nat Immunol. 0.975694444. PubMed Jacome-Galarza C, et al. 2011. J Bone Miner Res. 26:1207. PubMed Kotfis K, et al. 2020. British Journal of Pharmacology. 49(1):66-72. PubMed Jin RM, et al. 2017. J Immunol. 198:352. PubMed Xiao P, et al. 2019. J Exp Med. 216:337. PubMed Lagares D, et al. 2017. Sci Transl Med. 9:eaal3765. PubMed Deng Z, et al. 2017. Oncogene. 36:639. PubMed Kimball AS et al. 2019. Immunity. 51(2):258-271 . PubMed Korangath P, et al. 2020. Bio Protoc. 10:e3822. PubMed Minogue A, et al. 2014. Neurobiol Aging. 35:1442. PubMed Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed Zheng J, et al. 2020. bioRxiv. . PubMed Huixin Xu et al. 2018. EMBO molecular medicine. 10(9) pii: e8931. PubMed Mao FY, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:395. PubMed Geng S, et al. 2021. JCI Insight. 6:. PubMed Synn CB, et al. 2022. Clin Transl Immunology. 11:e1364. PubMed Morales-Mantilla DE, et al. 2022. Elife. 11:. PubMed Chen M, et al. 2021. Oncol Lett. 21:435. PubMed Elahi S, et al. 2020. Stem Cell Res. 43:101710. PubMed Gousopoulos E, et al. 2016. Am J Pathol. 186:2193-2203. PubMed Radjabova V, et al. 2015. J Immunol. 195: 3149 - 3159. PubMed Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed Patel S, et al. 2019. Viruses. 0.877083333. PubMed Mishra BB, et al. 2017. Nat Microbiol. 2:17072. PubMed Roy K, et al. 2019. Immunity. 50:616. PubMed Bradley KC, et al. 2019. Cell Rep. 28:245. PubMed Christ A, et al. 2018. Cell. 172:162. PubMed Zhao X, et al. 2022. Nat Protoc. 17:2240. PubMed Zuo W, et al. 2022. Cell Death Discov. 8:257. PubMed Wu M, et al. 2013. Proc Natl Acad Sci U S A. 110:9439. PubMed Weinheimer-Haus E, et al. 2014. PLoS One. 9:91355. PubMed Ericson J, et al. 2014. PLoS One. 9:108553. PubMed Shih Y, et al. 2015. JPEN J Parenter Enteral Nutr. . PubMed Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed Li Z, et al. 2018. Front Immunol. 9:347. PubMed He J, et al. 2019. J Clin Invest. 130. PubMed Yu L, et al. 2019. Elife. 8:e46677. PubMed Liang J, et al. 2020. Sci Adv. 6:eabc3646. PubMed Shen M, et al. 2022. Nat Cancer. 3:60. PubMed Flögel U, et al. 2021. Nat Commun. 12:5847. PubMed Covarrubias AJ, et al. 2020. Nat Metab. 1265:2. PubMed Zheng J, et al. 2020. Nature. . PubMed Kim P, et al. 2015. Infect Immun . 83: 2992 - 3002. PubMed Fischer KJ, et al. 2019. J Immunol. 202:2027. PubMed Challa TD, et al. 2020. Cell Reports. 30(10):3424-3433. PubMed Liu C et al. 2019. Immunity. 51(2):381-397 . PubMed Uderhardt S, et al. 2019. Cell. 177:541. PubMed Hermetet F, et al. 2019. Nat Commun. 10:523. PubMed Nagatake T, et al. 2018. Int Immunol. 30:471. PubMed Shevchenko MA, et al. 2018. J Immunol Res. 8:53. PubMed Simpson K, et al. 2012. J Immunol. 189:5533. PubMed Giampazolias E, et al. 2021. Cell. . PubMed Prizant H, et al. 2021. Cell Reports. 36:109523. PubMed Xu P, et al. 2020. Cancer Immunol Res. 8:1193. PubMed Robinett RA et al. 2018. Cell systems. 7(1):41-48 . PubMed Rebecca S Dickinson et al. 2017. Wellcome open research. 0.155555556 . PubMed Nuro-Gyina PK, et al. 2021. J Immunol. 207:868. PubMed Zeng Q, et al. 2022. iScience. 25:105151. PubMed Taya M, et al. 2020. Endocr Relat Cancer. 27:261. PubMed Hirata SI, et al. 2020. Allergy. 75:1939. PubMed Xu G, et al. 2020. Cell Rep. 32:108170. PubMed Winchell CG, et al. 2020. Front Immunol. 1.077083333. PubMed Li YT, et al. 2019. Elife. 8:e47642. PubMed Xueyang Yu et al. 2017. Immunity. 47(5):903-912 . PubMed Nagatake T, et al. 2018. J Allergy Clin Immunol. 142:470. PubMed Takaku S, et al. 2022. Evid Based Complement Alternat Med. 2022:6122955. PubMed Qin W, et al. 2022. Bio Protoc. 12:e4287. PubMed Wang W, et al. 2021. J Am Heart Assoc. 10:e019142. PubMed Shibuya M, et al. 2021. iScience. 24:103131. PubMed Gorina R, et al. 2014. J Immunol. 192:324. PubMed Nacer A, et al. 2014. PLoS Pathog. 10:1004528. PubMed Gamradt P, et al. 2016. PLoS Pathog. 12:e1006032. PubMed Varikuti S, et al. 2020. Br J Cancer. 122:1005. PubMed Thomas DG et al. 2018. Cell reports. 25(13):3774-3785 . PubMed Lai SM et al. 2018. Cell reports. 25(11):3099-3109 . PubMed Chen ST et al. 2019. Cell host & microbe. 25(4):602-616 . PubMed Liu YG, et al. 2019. FASEB J. 33:5018. PubMed Cartwright ANR, et al. 2021. Cancer Immunol Res. 9:470. PubMed LeBlond ND, et al. 2020. J Lipid Res. 61:1697. PubMed Makino A, et al. 2021. iScience. 24:103201. PubMed Gao Z, et al. 2022. J Inflamm Res. 15:3337. PubMed Njock MS, et al. 2022. J Extracell Vesicles. 11:e12228. PubMed Wei Y, et al. 2021. Endocrinology. 162(8):. PubMed Asadi Shahmirzadi A, et al. 2020. Cell Metabolism. 32(3):447-456.e6. PubMed Xu C, et al. 2020. Immunity. 53(2):417-428.e4. PubMed Zhu YP et al. 2018. Cell reports. 24(9):2329-2341 . PubMed van Geffen C, et al. 2021. Front Immunol. 12:695933. PubMed Gu H, et al. 2021. Elife. 10:. PubMed Issah Y, et al. 2021. Elife. 10:. PubMed Danzer H, et al. 2020. Elife. 9:00. PubMed Sparber F, et al. 2019. Cell Host Microbe. 25:389. PubMed Chao JL, et al. 2021. Cell Rep Med. 2:100399. PubMed Gong P, et al. 2022. Cell Death Discov. 8:466. PubMed Chen J, et al. 2014. Cell Res. 24:1050. PubMed Xu Y, et al. 2014. Proc Natl Acad Sci U S A. 111:6371. PubMed Willemze RA, et al. 2019. Mol Med. 25:01:00. PubMed Jain A, et al. 2020. Nat Immunol. 0.920138889. PubMed Nakamura K, et al. 2018. Cancer Cell. 33:634. PubMed Smith LK, et al. 2021. Elife. 10:. PubMed Schmit T, et al. 2022. Cell Rep. 38:110456. PubMed Lee GR, et al. 2021. JCI Insight. 6:. PubMed Stadtmann A, et al. 2015. J Immunol. 195: 1152-1161. PubMed Jia H, et al. 2016. J Immunol. 197: 859 - 871. PubMed Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed Katsuyama T, et al. 2021. Cell Reports. 36(1):109339. PubMed Tian T, et al. 2020. Cancer Immunol Res. 660:8. PubMed Li Z et al. 2018. Immunity. 49(4):640-653 . PubMed Lee EKS, et al. 2018. Cell Host Microbe. 23:121. PubMed Dyer DP et al. 2019. Immunity. 50(2):378-389 . PubMed Orr MT, et al. 2019. NPJ Vaccines. 4:1. PubMed Geng S, et al. 2019. Sci Adv. 5:eaav2309. PubMed Rappe JCF, et al. 2021. J Exp Med. 218:. PubMed Rivera CA, et al. 2022. Immunity. 55:129. PubMed Awida Z, et al. 2021. Int J Mol Sci. 23:. PubMed Caporarello N, et al. 2022. Nat Commun. 13:4170. PubMed Zhang H, et al. 2022. Commun Biol. 5:916. PubMed Singh N, et al. 2016. Dis Model Mech. 9: 885 - 897. PubMed Cojohari O, Peppenelli M, Chan G 2016. Blood. 128: 529 - 541. PubMed Buschor S, et al. 2017. PLoS Pathogens. 13(6):e1006476. PubMed Wang Y, et al. 2022. Nat Cell Biol. 24:230. PubMed Aggarwal N, et al. 2021. Cell Rep. 37:110170. PubMed Chuang LT, et al. 2021. Molecules. 26:. PubMed
RRID: AB_1236488 (BioLegend Cat. No. 127605) AB_1236494 (BioLegend Cat. No. 127606)
Structure: A 21-35 kD GPI-anchorded membrane protein
Distribution: Expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes. The monoclonal antibody RB6-8C5 recognizes both Ly-6G and Ly-6C.
Cell Type: Granulocytes, Macrophages, Monocytes
Biology Area: Immunology, Innate Immunity
Antigen References: Fleming TJ, et al. 1993. J. Immunol. 151:2399.
Gene ID: 546644
UniProt: View information about Ly-6G on UniProt.org
Clone: 1A8
Regulatory Status: RUO
Other Names: Lymphocyte antigen 6 complex, locus G
Isotype: Rat IgG2a, κ