Biolegend, 127608, PE anti-mouse Ly-6G Antibody, 200μg

Lymphocyte antigen 6 complex, locus G (Ly-6G), a 21-25 kD GPI-anchored protein, is expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes.
200μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Ly-6G transfected EL-4J cell line.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: While 1A8 recognizes only Ly-6G, clone RB6-8C5 recognizes both Ly-6G and Ly-6C. Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C15. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A815. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.416. Additional reported applications (for the relevant formats) include: immunohistochemistry9 of frozen sections10 and paraffin-embedded sections11, depletion4, 12-14, and spatial biology (IBEX)20,21. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for in vivo studies or highly sensitive assays (Cat. No. 127632, 127649, 127650, 127661 and 127662).
Application References(PubMed link indicates BioLegend citation): Fleming TJ, et al. 1993. J. Immunol. 151:2399. (FC) Daley JM, et al. 2008. J. Leukocyte Biol. 83:1. (FC) Dietlin TA, et al. 2007. J. Leukocyte Biol. 81:1205. (FC) Daley J, et al. 2007. J. Leukocyte Biol. doi:10.1189. (Deplete) PubMed Tadagavadi RK, et al. 2010. J. Immunol. 185:4904. PubMed Sumagin R, et al. 2010. J. Immunol. 185:7057. PubMed Guiducci C, et al. 2010. J. Exp Med. 207:2931. PubMed Fujita M, et al. 2011. Cancer Res. 71:2664. PubMed Van Leeuwen, et al. 2008. Arterioscler. Thromb. Vasc. Biol. 28:84. (IHC) Kowanetz M, et al. 2010. P. Natl. Acad. Sci. USA 107:21248. [supplementary data] (IHC) Esbona K, et al. 2016. Breast Cancer Res. 18:35. (IHC) Wojtasiak M, et al. 2010. J. Gen. Virol. 91:2158. (FC, Deplete) Jaeger BN, et al. 2012. J. Exp. Med. 209:565. (Deplete) Wozniak KL, et al. 2012. BMC Immunol. 13:65 (FC, Deplete) Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538. Ng LG, et al. 2011. J Invest. Dermatol. 131:2058. PubMed Ma C, et al. 2012. J. Leukoc. Biol. 92:1199. McCartney-Francis, N, et al. 2014. J Leukoc. Biol. 96:917. PubMed Her Z, et al. 2014. EMBO Mol. Med. 7:24. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Lee T, et al. 2014. Mol Biol Cell. 25:583. PubMed Hernández-Santana YE, et al. 2020. Life Sci Alliance. 3:00. PubMed D'Alessandro G, et al. 2020. Eur J Immunol. 50:705. PubMed DeSouza-Vieira T, et al. 2020. Cell Rep. 33:108317. PubMed Bowling S, et al. 2020. Cell. 181(6):1410-1422.e27. PubMed Combes F, et al. 2018. Neoplasia. 20:848. PubMed de la Rosa X, et al. 2018. Am J Pathol. 188:950. PubMed Ramakrishna C, et al. 2019. Nat Commun. 10:2153. PubMed Sierro F, et al. 2017. Immunity. 47:374. PubMed Yu X, et al. 2020. Nat Commun. 11:1110. PubMed Christian DA, et al. 2022. Sci Immunol. 7:eabq7432. PubMed Pagni RL, et al. 2022. Front Immunol. 13:1005937. PubMed Matter AL, et al. 2022. J Immunol. 209:1880. PubMed Mizutani T, et al. 2023. iScience. 26:106081. PubMed Wang JX, et al. 2023. JCI Insight. 8:. PubMed Zhu S, et al. 2023. J Nanobiotechnology. 21:81. PubMed Chung JY, et al. 2023. Nat Commun. 14:1794. PubMed Sun JY, et al. 2023. Sci Adv. 9:eade4110. PubMed Cozzolino M, et al. 2023. Int J Mol Sci. 24:. PubMed Kase N, et al. 2023. Front Cell Dev Biol. 11:1022081. PubMed Yang QC, et al. 2023. iScience. 26:106916. PubMed Roy S, et al. 2023. J Clin Invest. 133:. PubMed Guo H, et al. 2020. Curr Protoc Immunol. 131:e107. PubMed Liang J, et al. 2021. Cancer Manag Res. 13:6977. PubMed Zhao D, et al. 2021. Innate Immun. 27:533. PubMed Zhou R, et al. 2022. EBioMedicine. 75:103762. PubMed Volmari A, et al. 2021. Hepatol Commun. 5:2104. PubMed Da Mesquita S, et al. 2021. Nature. 593:255. PubMed Bettke JA, et al. 2022. Infect Immun. 90:e0007022. PubMed MacLean AJ, et al. 2022. Immunity. 55:718. PubMed Liu X, et al. 2022. Adv Sci (Weinh). 9:e2105650. PubMed Mirchandani AS, et al. 2022. Nat Immunol. 23:927. PubMed Xu J, et al. 2022. Invest Ophthalmol Vis Sci. 63:15. PubMed Wu X, et al. 2022. J Clin Med. 11: . PubMed Gong F, et al. 2023. Adv Healthc Mater. 12:e2201771. PubMed Hopke A, et al. 2022. iScience. 25:105226. PubMed Nagre N, et al. 2022. Respir Res. 23:326. PubMed Shang T, et al. 2022. Front Pharmacol. 13:1054176. PubMed Wang Y, et al. 2023. FASEB J. 37:e22776. PubMed Pinto NA, et al. 2023. Cell Death Differ. :. PubMed Meng S, et al. 2023. Stem Cell Investig. 10:2. PubMed He Y, et al. 2023. Nat Commun. 14:691. PubMed Zhong CS, et al. 2022. Acta Pharmacol Sin. 43:1324. PubMed De Giovanni M, et al. 2022. Cell. 185:815. PubMed Ma Y, et al. 2022. Am J Physiol Heart Circ Physiol. 322:H622. PubMed Kraynak CA, et al. 2022. Int J Pharm. 618:121634. PubMed Cai B, et al. 2016. Proc Natl Acad Sci U S A. 113: 6526 - 6531. PubMed Kozik J, et al. 2016. Sci Rep. 6:28556. PubMed Herz J, et al. 2015. J Exp Med. 212: 1153 - 1169. PubMed Jackson A, et al. 2014. J Leukoc Biol. 92:609. PubMed Jung J, et al. 2019. Cell Host Microbe. 25:513. PubMed Fachi JL et al. 2019. Cell reports. 27(3):750-761 . PubMed Alissafi T, et al. 2018. J Clin Invest. 128:3840. PubMed Iwamoto H et al. 2018. Cell metabolism. 28(1):104-117 . PubMed Gawish R, et al. 2022. Elife. 11:. PubMed Li X, et al. 2022. Nat Commun. 13:2794. PubMed He J, et al. 2022. Front Immunol. 12:760138. PubMed Ancey PB, et al. 2021. Cancer Res. 81:2345. PubMed Machata S, et al. 2021. Front Immunol. 11:565869. PubMed Tang L, et al. 2021. Oncol Lett. 22:605. PubMed Ajendra J, et al. 2016. Sci Rep. 6:39648. PubMed Bottek J, et al. 2020. Mucosal Immunol. 1.029166667. PubMed Sharma K, et al. 2021. Cell Reports. 36(3):109351. PubMed Zhang Y, et al. 2018. Sci Rep. 8:5994. PubMed Kenichi Shimada et al. 2018. Immunity. 49(5):873-885 . PubMed Zhang K, et al. 2018. PLoS Pathog. 14:e1006925. PubMed Delás MJ, et al. 2019. Cell Rep. 27:719. PubMed Putnam NE, et al. 2019. PLoS Pathog. 15:e1007744. PubMed Hanhai Z, et al. 2021. Aging (Albany NY). 13:13108. PubMed Choi YY, et al. 2021. Cell Death Dis. 12:826. PubMed Acharya N, et al. 2020. Immunity. 53(3):658-671.e6. PubMed Clement M, et al. 2016. PLoS Pathog. 12:e1006050. PubMed Montufar-Solis D, Klein J 2016. PLoS One. 11: 014611. PubMed Sun P, et al. 2015. J Hepatol. . PubMed Okada M, et al. 2014. J Biol Chem. 289:32926. PubMed Lechner AJ et al. 2017. Cell stem cell. 21(1):120-134 . PubMed Ishii T,et al. 2017. Sci Rep.. 10.1038/s41598-017-12513-1. PubMed Xie D, et al. 2017. PLoS One. 10.1371/journal.pone.0181454. PubMed Muhammad W, et al. 2022. Acta Biomater. 148:258. PubMed Rogic A, et al. 2021. Nat Commun. 12:6889. PubMed Harding M, et al. 2014. PLoS Pathog. 10:1004379. PubMed Morales D, et al. 2015. J Virol. 89:337. PubMed Lin T, et al. 2015. PLoS One. 10: e0138335. PubMed Iwata H, et al. 2016. Nat Commun. 7:12849. PubMed Yeo WS, et al. 2020. J Bacteriol. 202:00:00. PubMed Webster HC, et al. 2020. J Immunol Methods. 112702:477. PubMed Pham THM, et al. 2020. Cell Host & Microbe. 27(1):54-67.e5.. PubMed Go DM, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:715. PubMed Bae SH, et al. 2021. Theranostics. 11:2522. PubMed Zhou HF, et al. 2022. Front Pharmacol. 13:778755. PubMed Helm M, et al. 2022. Life (Basel). 12:. PubMed Dolgova EV, et al. 2022. Int J Mol Sci. 23:. PubMed Papafragkos I, et al. 2022. Front Immunol. 13:889075. PubMed Lemon J, et al. 2015. Infect Immun . 83: 3204-3212. PubMed Zhou Z, et al. 2019. Oxid Med Cell Longev. 2019:1209307. PubMed Mastandrea I, et al. 2022. STAR Protoc. 3:101106. PubMed Ma M, et al. 2021. Front Immunol. 12:718779. PubMed Zhang Z, et al. 2021. Oncoimmunology. 10:1912472. PubMed Metzger D, et al. 2015. J Infect Dis. 212: 445-452. PubMed Winkler MJ, et al. 2020. Basic Res Cardiol. 115:67. PubMed Ku MW, et al. 2020. Cell Host Microbe. 29(2):236-249.e6. PubMed Ratitong B, et al. 2021. Cell Reports. 35(7):109139. PubMed Müller A, et al. 2020. J Clin Invest. 5765:130. PubMed Fujita Y et al. 2018. Cell reports. 24(12):3296-3311 . PubMed Schaffert A, et al. 2020. Front Immunol. 10:3024. PubMed Galvão I, et al. 2018. Front Immunol. 9:142. PubMed Shin SH, et al. 2022. Front Cell Neurosci. 16:844480. PubMed Zhang J, et al. 2022. iScience. 25:104963. PubMed Qiu Z, et al. 2016. Sci Rep. 6:31936. PubMed Kojo K, et al. 2016. Sci Rep. 6:29650. PubMed Hegde S, et al. 2020. Cancer Cell. 37(3):289-307. PubMed Al Mamun A, et al. 2019. Neurochem Int. 127:148. PubMed Deng Z, et al. 2017. Oncogene. 36:639. PubMed Varol D et al. 2017. Immunity. 46(6):1030-1044 . PubMed McLaughlin PA et al. 2019. PLoS Pathog. 15(7):e1007847 . PubMed Linde N, et al. 2018. Nat Commun. 9:21. PubMed Soto-Díaz K, et al. 2021. Front Immunol. 12:734349. PubMed El-Zaatari M, et al. 2014. J Immunol. 193:807. PubMed Hulsmans M et al. 2017. Cell. 169(3):510-522 . PubMed Si Y, et al. 2018. J Control Release. 282:120. PubMed Du Y, et al. 2022. Nat Commun. 13:231. PubMed Catani J, et al. 2016. Transl Oncol. 9:565-574. PubMed Markman JL, et al. 2020. Nat Commun. 11:1613. PubMed Wheeler MA, et al. 2019. Cell. 176:581. PubMed Steuerman Y et al. 2018. Cell systems. 6(6):679-691 . PubMed Zhou Z, et al. 2022. Life (Basel). 12:. PubMed Baik JE, et al. 2022. Clin Transl Med. 12:e758. PubMed Yong L, et al. 2022. Nat Commun. 13:4255. PubMed Coquery C, et al. 2014. PLoS One. 9:102284. PubMed Umeshappa CS, et al. 2021. Cell Reports. 34(13):108919. PubMed Abe M, et al. 2020. Clin Exp Immunol. 303:199. PubMed Zhou G, et al. 2019. FASEB J. 33:11396. PubMed Cosentino K, et al. 2022. Mol Cell. 82:933. PubMed Su M, et al. 2022. Nat Cardiovasc Res. 1:732. PubMed Frenis K, et al. 2021. Basic Res Cardiol. 31:116. PubMed Unudurthi SD, et al. 2020. Life Sci. 117440:247. PubMed Finsterbusch M, et al. 2014. J Exp Med. 211:1307. PubMed Vergadi E, et al. 2014. J Immunol. 192:394. PubMed Kim Y, et al. 2012. J Immunol. 188:4226. PubMed Yamada KJ, et al. 2020. PLoS Pathog. 16:e1008354. PubMed Rynkiewicz NK, et al. 2020. Sci Signal. 13:. PubMed Gopinath A, et al. 2022. NPJ Parkinsons Dis. 8:72. PubMed Sanmarco LM, et al. 2021. Nature. 590:473. PubMed Daneshmandi S, et al. 2021. Elife. 10:. PubMed Tapia-Abellán A, et al. 2021. Sci Adv. 7:eabf4468. PubMed Jiang Y, et al. 2021. Cell Death Differ. 28:3009. PubMed Uematsu T, et al. 2016. Sci Rep. 6:37815. PubMed Takesue S, et al. 2020. Int J Oncol. 56:596. PubMed Kimura H, et al. 2019. Sci Rep. 9:5887. PubMed Hsu BE et al. 2019. Cell Rep. 27(13):3902-3915 . PubMed Su W et al. 2019. Cancer Cell. 36(2):139-155 . PubMed Bronisz–Budzynska I, et al. 2019. Skelet Muscle. 9:22. PubMed Gamrekelashvili J, et al. 2021. Bio Protoc. 11:e4007. PubMed Takizawa S, et al. 2022. Mol Med. 28:91. PubMed Ryan NM, et al. 2022. Front Immunol. 13:932742. PubMed Wang G, et al. 2021. Cell Host Microbe. 29(5):777-791.e6. PubMed Swanson P, et al. 2016. PLoS Pathog. 12:e1006022. PubMed Kim M, et al. 2018. Immunity. 49:151. PubMed Petkevicius K, et al. 2019. Elife. 8:e47990. PubMed Baomei Wang et al. 2019. Cell reports. 26(6):1614-1626 . PubMed Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed Murray MP, et al. 2022. Cell Rep. 38:110209. PubMed Zhou J, et al. 2022. Int J Oncol. 61: . PubMed Wang W, et al. 2021. J Am Heart Assoc. 10:e019142. PubMed Penke LR, et al. 2020. Life Sci Alliance. 3:. PubMed Liu Y, et al. 2015. BioMed Res Int. 2015 563425. PubMed Jiang L, et al. 2020. Cell. 183(5):1219-1233.e18. PubMed Onai N, et al. 2020. Int J Mol Sci. :22. PubMed Wang T et al. 2018. Immunity. 49(3):504-514 . PubMed Laban H, et al. 2018. J Cell Biol. 217:1503. PubMed Chiang N, et al. 2019. J Clin Invest. 129:5294. PubMed Eislmayr K, et al. 2022. Sci Adv. 8:eabj7293. PubMed Unsinger J, et al. 2021. J Leukoc Biol. 109:697. PubMed Vaibhav K, et al. 2020. Sci Adv. 6:eaax8847. PubMed Huang W, et al. 2016. Cell Death Differ. 10.1038/cdd.2016.115. PubMed Buerfent BC, et al. 2019. GMS Infect Dis. 7:Doc04. PubMed Grayczyk JP et al. 2017. Cell host & microbe. 22(5):678-687 . PubMed Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed Zhu YP et al. 2018. Cell reports. 24(9):2329-2341 . PubMed Sadhu S, et al. 2021. J Immunol. 207:1812. PubMed Rao L, et al. 2020. Nat Commun. 11:4909. PubMed Ding R, et al. 2022. J Neuroinflammation. 19:137. PubMed Zhang W, et al. 2021. Front Immunol. 12:768435. PubMed Zheng Y, et al. 2022. J Immunol. 208:501. PubMed Issah Y, et al. 2021. Elife. 10:. PubMed Kiss M, et al. 2020. Cancer Immunol Res. 9:309. PubMed Kimura T, et al. 2014. Int Immunol. 26:697. PubMed Fujimura N, et al. 2016. Sci Rep. 5:11664. PubMed Hu R, et al. 2015. J Immunol. 195: 4802 - 4809. PubMed Zhang T, et al. 2020. JCI Insight. 5:00. PubMed Huang WC, et al. 2020. Adv Mater. 32:e2005637. PubMed Matsumoto M, et al. 2021. Cell Host Microbe. 29(6):930-940.e4. PubMed Porrello A, et al. 2018. Nat Commun. 9:1988. PubMed Kong XF, et al. 2018. Nat Immunol. 19:973. PubMed Weckbach LT, et al. 2019. J Exp Med. 216:350. PubMed Subramaniam N, et al. 2021. Blood Adv. 5:1259. PubMed Li Y, et al. 2022. Theranostics. 12:5364. PubMed Zhai T, et al. 2020. Clin Exp Pharmacol Physiol. 47:677. PubMed Xu C, et al. 2021. Cell Reports. 35(11):109235. PubMed Martins R, et al. 2016. Nat Immunol. 17:1361-1372. PubMed Guo H, et al. 2016. J Immunol. 196: 2300 - 2308. PubMed Li Y, et al. 2020. Theranostics. 10:11376. PubMed Nguyen HX et al. 2017. J Neurosci. 37(38):9269-9287 . PubMed Moraes JA, et al. 2018. J Innate Immun. 11:330. PubMed Zhao Z, et al. 2019. J Immunol Res. 2019:9561350. PubMed Winter C, et al. 2018. Cell Metab. 28:175. PubMed Hirota K et al. 2018. Immunity. 48(6):1220-1232 . PubMed Ishihara J, et al. 2018. Nat Commun. 9:2163. PubMed Bozoglu T, et al. 2022. Adv Sci (Weinh). 9:e2103867. PubMed Chen YL, et al. 2022. Front Neurosci. 16:876582. PubMed Hou Y, et al. 2013. J Nutr Biochem. 24:1555. PubMed Zhang S, et al. 2016. PLoS Pathog. 12: 1005617. PubMed Wang J, et al. 2020. Cell. 183(7):1867-1883.e26. PubMed Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed Wang X, et al. 2021. Immunity. 54(6):1123-1136.e8. PubMed Schuran FA, et al. 2020. Cell Mol Gastroenterol Hepatol. . PubMed Wheeler MA, et al. 2020. Nature. 578:593. PubMed Wang C, et al. 2020. Mucosal Immunol. 13:22. PubMed Weng JH, et al. 2021. Nat Metab. 3:513. PubMed Shin SH, et al. 2022. Clin Exp Otorhinolaryngol. . PubMed Zha ZX, et al. 2022. Acta Pharmacol Sin. :. PubMed Jia J, et al. 2022. Nat Commun. 13:6804. PubMed Zhang B, et al. 2021. Front Neurol. 607370:12. PubMed Uematsu T, et al. 2015. Sci Rep. 5: 17577. PubMed Rodgers M, et al. 2014. J Exp Med. 211:1333. PubMed Hellmann J, et al. 2013. J Immunol. 191:1383. PubMed Ma Y, et al. 2020. Front Immunol. 11:586685. PubMed Pessoa Rodrigues C, et al. 2020. Sci Adv. 6:eaaz4815. PubMed Thomson CA, et al. 2018. J Immunol. 201:215. PubMed Ribechini E, et al. 2017. Blood Adv. 0.699305556. PubMed Qu J, et al. 2021. Int J Biol Sci. 17:2756. PubMed
RRID: AB_1186104 (BioLegend Cat. No. 127607) AB_1186099 (BioLegend Cat. No. 127608)
Structure: A 21-35 kD GPI-anchorded membrane protein
Distribution: Expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes. The monoclonal antibody RB6-8C5 recognizes both Ly-6G and Ly-6C.
Cell Type: Granulocytes, Macrophages, Monocytes
Biology Area: Immunology, Innate Immunity
Antigen References: Fleming TJ, et al. 1993. J. Immunol. 151:2399.
Gene ID: 546644
UniProt: View information about Ly-6G on UniProt.org
Clone: 1A8
Regulatory Status: RUO
Other Names: Lymphocyte antigen 6 complex, locus G
Isotype: Rat IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.