Biolegend, 127824, Alexa Fluor® 700 anti-mouse TCR Vα2 Antibody, 100μg

The TCR alpha (α) chain complexes with the TCR beta (β) chain to form the T cell receptor in 95% of T cells, whereas the remaining 5% of T cells express gamma and delta chains (γ/δ).  TCR Vα2 is a distinct TCR subfamily found in mice having the a, b, and c haplotypes.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Soluble TCR from mouse CTL clone KB5-C20
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: The B20.1 antibody recognizes most members of the Va2 TCR subfamily in mice having the a, b, and c haplotypes.
Application References(PubMed link indicates BioLegend citation): Pircher H, et al. 1992. Eur. J. Immunol.. 22:399. Gregoire C, et al. 1991. P. Natl. Acad. Sci. USA 88:8077. Kao C, et al. 2005. Int. Immunol.17:1607. PubMed Steptoe RJ, et al. 2007. J. Immunol. 178:2094. PubMed Rao RR, et al. 2012. Immunity. 36:374. PubMed.
Product Citations: Iberg CA, et al. 2022. Cell Rep. 39:110657. PubMed Li CY, et al. 2022. Int J Mol Sci. 23:. PubMed
RRID: AB_2814018 (BioLegend Cat. No. 127823) AB_2814019 (BioLegend Cat. No. 127824)
Distribution: On a subset of T cells
Function: In combination of V β, Vα recognizes MHC/peptide complex on the antigen-presenting cells.
Interaction: Associate with Vβ and CD3 complex to form TCR complexes expressing on cell surface.
Ligand/Receptor: TCR recognizes MHC/peptide complex
Cell Type: T cells
Biology Area: Adaptive Immunity, Immunology
Molecular Family: TCRs
Antigen References: 1. Kubo RT, et al. 1989. J. Immunol.. 142:2736. 2. Pircher H, et al. 1992. Eur. J. Immunol. 22:399.
Gene ID: 21473
UniProt: View information about TCR Valpha2 on UniProt.org
Clone: B20.1
Regulatory Status: RUO
Other Names: T cell receptor Vα 2
Isotype: Rat IgG2a, λ