Biolegend, 127907, PE anti-mouse TCR Vβ2 Antibody, 25μg

The B20.6 antibody recognizes V beta 2 TCR of all strains tested. V beta 2 bearing T lymphocytes are clonally eliminated in MHC class II I-E expressing mice neonatally infected with MMTV virus through milk feeding.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Soluble TCR from mouse CTL clone CW3/1.1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): Necker A, et al. 1991. Eur J. Immunol. 21:3035 Hodes RJ, et al. 1993. J Immunol 150:1422
Product Citations: Lutes LK, et al. 2021. eLife. 10:00. PubMed Zhang F, et al. 2019. Nat Commun. 10:3974. PubMed Nelson CE et al. 2019. Cell Rep. 28(12):3092-3104 . PubMed
RRID: AB_1227785 (BioLegend Cat. No. 127907) AB_1227784 (BioLegend Cat. No. 127908)
Structure: TCR has a two chain structure, consisting of alpha and beta chain. It belongs to Ig superfamily.
Distribution: On a subset of T cells
Function: Recognize MHC/peptide complex on the antigen-presenting cells.
Interaction: Associate with V alpha and CD3 complex to form TCR complexes expressing on cell surface.
Ligand/Receptor: TCR recognizes MHC/peptide complex
Cell Type: T cells
Biology Area: Adaptive Immunity, Immunology
Molecular Family: TCRs
Antigen References: 1. Kubo RT, et al. 1989. J. Immunol. 142:2736 2. Necker A, et al. 1991. Eur. J. Immunol. 21:3035
Gene ID: 21577
UniProt: View information about TCR Vbeta2 on UniProt.org
Clone: B20.6
Regulatory Status: RUO
Other Names: T cell receptor Vβ 2 [vb2]
Isotype: Rat IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.