Biolegend, 127910, Alexa Fluor® 647 anti-mouse TCR Vβ2 Antibody, 100μg

The B20.6 antibody recognizes V beta 2 TCR of all strains tested. V beta 2 bearing T lymphocytes are clonally eliminated in MHC class II I-E expressing mice neonatally infected with MMTV virus through milk feeding.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Soluble TCR from mouse CTL clone CW3/1.1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application References(PubMed link indicates BioLegend citation): Necker A, et al. 1991. Eur J. Immunol. 21:3035 Hodes RJ, et al. 1993. J Immunol 150:1422
Product Citations: Waight JD, et al. 2018. Cancer Cell. 33:1033. PubMed
RRID: AB_1089254 (BioLegend Cat. No. 127910)
Structure: TCR has a two chain structure, consisting of alpha and beta chain. It belongs to Ig superfamily.
Distribution: On a subset of T cells
Function: Recognize MHC/peptide complex on the antigen-presenting cells.
Interaction: Associate with V alpha and CD3 complex to form TCR complexes expressing on cell surface.
Ligand/Receptor: TCR recognizes MHC/peptide complex
Cell Type: T cells
Biology Area: Adaptive Immunity, Immunology
Molecular Family: TCRs
Antigen References: 1. Kubo RT, et al. 1989. J. Immunol. 142:2736 2. Necker A, et al. 1991. Eur. J. Immunol. 21:3035
Gene ID: 21577
UniProt: View information about TCR Vbeta2 on UniProt.org
Clone: B20.6
Regulatory Status: RUO
Other Names: T cell receptor Vβ 2 [vb2]
Isotype: Rat IgG2a, κ