Biolegend, 128505, Alexa Fluor® 647 anti-mouse JAML Antibody, 25μg

JAML is a type I integral transmembrane protein with 2 extracellular IgSF domains characteristic of many proteins implicated in cell to cell interactions.JAML is primarily express on granulocytes and reported to be involved in neutrophil migration across tight junctions by its adhesive interaction with epithelial Coxsackie and adenovirus receptor (CAR).
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Armenian Hamster
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for other applications.* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application References(PubMed link indicates BioLegend citation): Zen K, et al. 2005. Mol Biol Cell 16:2694
Product Citations: Edwards SC, et al. 2023. J Exp Med. 220: . PubMed Tan L, et al. 2019. Cell Rep. 27:3657. PubMed
RRID: AB_1186014 (BioLegend Cat. No. 128506)
Structure: Type I integral transmembrane protein with 2 extracellular IgSF domains characteristic of proteins implicated in cell-cell interactions, a transmembrane segament and an intracellular tail.
Distribution: Primarily on granulocytes
Ligand/Receptor: Epithelial Coxsackie and adenovirus receptor (CAR)
Cell Type: Granulocytes, Neutrophils
Biology Area: Immunology
Molecular Family: Adhesion Molecules
Antigen References: 1. Moog-Lutz C et al. 2003. Blood 102 :3371
Gene ID: 270152
UniProt: View information about JAML on UniProt.org
Clone: 4E10
Regulatory Status: RUO
Other Names: AMICA
Isotype: Armenian Hamster IgG