Biolegend, 128710, PE anti-mouse CD199 (CCR9) Antibody, 100μg

CCR9 is a member of the G protein coupled receptor family and is involved in T cell development in the thymus and in gut-associated immune response. Mouse CCR9 is highly expressed on different stages of thymocytes and upregulated on CD4+CD8+ cells. It is expressed to a much lower level on spleen and lymph node cells. Expression of CCR9 on γ/δ T cells in the intraepithelial and small intestine has been reported. The interaction of CCR9 with its ligand CCL25 (TECK, thymus-expressed chemokine) may direct the trafficking of developing T cells in the thymus and generation of gut-specific immunological memory.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations: Beura LK, et al. 2018. Immunity. 48:327. PubMed
RRID: AB_1227479 (BioLegend Cat. No. 128709) AB_1227478 (BioLegend Cat. No. 128710)
Structure: A membrane of G-protein coupled receptors and contains seven transmembrain domains. Mouse CCR9 has above 80% amino acid sequence homology with human counterpart.
Distribution: CD4+CD8+ thymocytes, γ/δ T cells
Interaction: CCL25
Ligand/Receptor: CCL25
Cell Type: T cells, Thymocytes
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References: Zaballos A, et al. 1999. J. Immunol. 162:5671. Wurbel MA, et al. 2007. J. Immunol. 178:7598. Wurbel MA, et al. 2006. Eur. J. Immunol. 36:73.
Gene ID: 12769
UniProt: View information about CD199 on UniProt.org
Clone: CW-1.2
Regulatory Status: RUO
Other Names: CCR9, GPR-9-6,GPR28, CDw199
Isotype: Mouse IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.