Biolegend, 128808, Alexa Fluor® 647 anti-mouse CD226 (DNAM-1) Antibody, 100μg

CD226 (DNAM-1) is constitutively expressed on native CD8 + cells and on CD4 + T cells, macrophages and NK cells.This antibody (10E5) was reported to bind about 40% of inactivated CD4 + cells and binds only to differentiated Th1 cells, but not to Th2 or Th0 cells.It is also reported to suppress antigen-specific T cell expansion and EAE (experimental allergic encephalitis) mediated by Th1 cells.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Th1 polarized T cell clones
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application References(PubMed link indicates BioLegend citation): Dardalhon V, et al. 2005. J. Immunol. 175:1558 Huang Z, et al. 2011. J Immunol. 186:3421. PubMed
Product Citations: Shapiro MR, et al. 2020. Front Immunol. 1.972222222. PubMed Berglund L, et al. 2013. Blood. 122:3940. PubMed Wagner AK, et al. 2022. iScience. 25:105137. PubMed
RRID: AB_1227541 (BioLegend Cat. No. 128808)
Structure: 65 kd glycoprotein of a member of Ig-superfamily containing 2 immunologlobulin-like domains.
Distribution: Expressed on majority of CD4+ and CD8+ T cells, NK cells, monocytes/macrophages and a subset of B cells and thymocytes. Reported to be specifically expressed on the surface of Th1 cells, but downregulated in Th2 cells.
Function: Mediates cellular adhesion, involved in LFA-1 costimulatory signal for T cell differentiation and proliferation
Ligand/Receptor: CD112 and CD155
Cell Type: B cells, Macrophages, Monocytes, NK cells, T cells, Th1
Biology Area: Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1.Tahara Hanaoka S, et al. 2006. Blood 107:1491 2. Shibuya K, et al. 2003. J. Exp. Med. 198:1829
Gene ID: 225825
UniProt: View information about CD226 on UniProt.org
Clone: 10E5
Regulatory Status: RUO
Other Names: DNAM-1, PTA1 (platelet and T cell activation antigen 1), TLISA1, LFA-1 associated Molecule PTA-1
Isotype: Rat IgG2b, κ