Biolegend, 129605, PE anti-mouse Siglec H Antibody, 25μg

The Siglec-H is a member of CD33-related Siglec family which mostly expressed in the innate immune system. Siglec-H is reported to be specifically expressed on plasmacytoid dendritic cells. Siglec-H depends on adaptor protein DAP 12 (DNAX-activating protein of 12 kD) for surface expression and signal transduction. Cross-linking with this antibody can selectively inhibit interferon-alpha production by IPCs following TLR9 ligation.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Siglec-H-Fc recombinant fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): Blasius AL, et al. 2006. J. Immunol. 177:3260.
Product Citations: Griffin GK, et al. 2023. Nature. 618:834. PubMed Doughty D, et al. 2022. Exp Biol Med (Maywood). 247:1433. PubMed Miller CM, et al. 2020. J Virol. 94:00:00. PubMed Furuta Y, et al. 2017. PLoS One. 12(2):e0172509. PubMed Mogilenko DA, et al. 2020. Immunity. 54(1):99-115.e12. PubMed Müller A, et al. 2020. J Clin Invest. 5765:130. PubMed Gozgit JM, et al. 2021. Cancer Cell. :. PubMed Leylek R, et al. 2019. Cell Rep. 29:3736. PubMed Brown CC, et al. 2020. Cell. 179(4):846-863.e24.. PubMed Bradley KC, et al. 2019. Cell Rep. 28:245. PubMed Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed Li C, et al. 2021. Cell Metabolism. 33(8):1610-1623.e5. PubMed Shiozawa S, et al. 2022. iScience. 25:103537. PubMed Youshani AS, et al. 2019. J Neuroinflammation. 16:25. PubMed Dyer DP et al. 2019. Immunity. 50(2):378-389 . PubMed Ioanna E Galani et al. 2017. Immunity. 46(5):875-890 . PubMed Rappe JCF, et al. 2021. J Exp Med. 218:. PubMed Uematsu T, et al. 2015. Sci Rep. 5: 17577. PubMed
RRID: AB_1227763 (BioLegend Cat. No. 129605) AB_2189147 (BioLegend Cat. No. 129606)
Structure: Sialic acid-binding immunoglobulin-like lectins
Distribution: Siglec-H is expressed exclusively on natural interferon-producing cells (IPCs), also called plasmacytoid dendritic cells in spleen, blood and lymph nodes. The IPCs are characterized by a complex phenotype (CD11c+, Ly-6C+, B220+, CD11b-, CD8+/-).
Function: Siglec-H associates with adaptor protein DAP12 for cell signaling, which reduces type 1 IFN response of IPC to CpG.
Ligand/Receptor: Sialic acid
Cell Type: Dendritic cells
Biology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules, Siglec Molecules
Antigen References: 1. Blasius AL, et al. 2006. Blood 107:2474. 2. Zhang J, et al. 2006. Blood 107:3600. 3. Blasius AL, et al. 2007. J. Exp. Med. 204:2561.
Gene ID: 233274
UniProt: View information about Siglec H on UniProt.org
Clone: 551
Regulatory Status: RUO
Other Names: Sialic acid-binding immunoglobulin-like lectin, SiglecH
Isotype: Rat IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.