Biolegend, 129810, PerCP/Cyanine5.5 anti-mouse CD196 (CCR6) Antibody, 100μg

CCR6 is a G-protein linked chemokine receptor which binds the chemokine CCL20/MIP-3a. It is expressed on B lymphocytes and certain subsets of dendritic cells and T cells. CCR6 is reported to be involved in mucosal immune response and lymphocyte migration and homeostasis.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Armenian Hamster
Immunogen: N-terminal peptide (aa. 2-38) of mouse CCR6 fused with GST
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Product Citations: Cortés JR, et al. 2022. Cell Rep. 39:110695. PubMed Zhan CS, et al. 2020. FASEB J. 14006:34. PubMed He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed Ghilas S, et al. 2021. iScience. 24:103059. PubMed Pardo E, et al. 2017. PLoS One. 12(6):e0177472. PubMed Lind L, et al. 2021. J Neurovirol. 27:145. PubMed Goode D, et al. 2014. PLoS Pathog. 10:1004567. PubMed
RRID: AB_2072797 (BioLegend Cat. No. 129809) AB_2275515 (BioLegend Cat. No. 129810)
Structure: G-protein linked, seven transmembrane, domain-spanning chemokine receptor; 367 amino acids long and about 75% homology with human CCR6
Distribution: Expressed on spleen B cells, immature splenic dendritic cells in Peyer's patches, and some T cell subset
Function: Important in regulating mucosal immune response
Ligand/Receptor: CCL20/MIP-3a
Cell Type: B cells, Dendritic cells, T cells
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References: 1. Rossi D, et al. 1997. J. Immunol. 158:1033. 2. Lukacs N, et al. 2001. J. Exp. Med. 194:551. 3. Cook DN, et al. 2000. Immunity 12:495
Gene ID: 12458
UniProt: View information about CD196 on UniProt.org
Clone: 29-2L17
Regulatory Status: RUO
Other Names: CD196, BN-1, CKR-L3, DRY6, KY411, STRL22
Isotype: Armenian Hamster IgG
Q: Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?
A: Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.