Biolegend, 130201, Purified anti-mouse CD314 (NKG2D) Antibody, 50μg

This antibody has been reported to block binding of NKG2D to its ligands, RAE-1 and H60, and inhibition of NKG2D-dependent NK-cell cytotoxicity.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse NKG2D
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: To reduce non-specific binding, preincubate cells with purified anti-mouse CD16/CD32, clone 93 (Cat No 101301/2) is recommended. This product is for in vitro research use only. It is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes contact The Regents of the University of California.
Application References(PubMed link indicates BioLegend citation): Ogasawara K, et al. 2003. Immunity 18:41. (Block) Ogasawara K, et al. 2004. Immunity 20:757. (Block) Kong LY, et al. 2010. Clin. Cancer Res. 16:2550. PubMed
Product Citations: Potempa M, et al. 2022. J Immunol. 208:1742. PubMed Goh W, et al. 2020. Cell Rep. 33:108285. PubMed
RRID: AB_1227717 (BioLegend Cat. No. 130202)
Structure: NKG2D is a lectin-like type II transmembrane protein. It serves as a stimulatory receptor to activate NK cells via the non-covalently associated DAP10 or DAP 12 daptor protein.
Distribution: NK, NKT cells
Ligand/Receptor: RAE, H60,
Cell Type: NK cells, NKT cells
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Gene ID: 27007
UniProt: View information about CD314 on UniProt.org
Clone: CX5
Regulatory Status: RUO
Other Names: CD314
Isotype: Rat IgG1, κ