Biolegend, 131509, APC anti-mouse CD155 (PVR) Antibody, 25μg

Mouse CD155, also known as homolog of polivirus receptor (PVR), is the founding member of a subfamily of immunoglobulin-like adhesion receptors (nectins). Mouse CD155 is expressed on a wide variety of cells of hematopoietic origin apart from its existence on the epithelial cells. The engagement of CD155 with CD226 and CD96 induces cytotoxicity of NK cells and CTL.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: CD155 transfectants
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application References(PubMed link indicates BioLegend citation): Tahara-Hanaoka S, et al. 2004. Int. Immunol. 16:533. Maier MK, et al. 2007. Eur. J. Immunol.. 37:2214. Bottino C, et al. 2003. J. Exp. Med. 198:557. Dressel R, et al. 2010. FASEB J. 9:134. PubMed
Product Citations: Du X, et al. 2018. Proc Natl Acad Sci U S A. 115:E11731. PubMed Wang N, et al. 2020. Front Immunol. 1.765972222. PubMed
RRID: AB_10640453 (BioLegend Cat. No. 131509) AB_10645507 (BioLegend Cat. No. 131510)
Structure: 70 kd immunoglobulin-like molecule with three Ig-like domains.
Distribution: CD155 is expressed at cell junctions on the primary vascular endothelial cells. Moreover, mouse CD155 is highly expressed on DP thymocytes and expression level is dropped in peripheral single positive CD4+ and CD8+ T cells. CD155 can be detected on T reg, other activated T cells and on NKT cells. Resident monocytes express moderate amount of CD155, but upregulated its expression during inflammation. Other cells, such as IEL, neutrophils and subsets of B cells were found to express CD155 to varying extents.
Function: Apart from its function of adhering junction among contacting epithelial cells, the interaction of DNAM-1 (CD226) with its ligands CD155 and CD112 (nectin 2) induces cytotoxcity of NK cells and CD8+ T cells and cytokine secretion.
Ligand/Receptor: CD226 (DNAM-1), CD96
Cell Type: B cells, Endothelial cells, Neutrophils, Thymocytes, Tregs
Biology Area: Immunology
Molecular Family: Adhesion Molecules, CD Molecules, Immune Checkpoint Receptors
Antigen References: 1. Mendelsohn C, et al. 1989. Cell 56:855.
Gene ID: 52118
UniProt: View information about CD155 on UniProt.org
Clone: TX56
Regulatory Status: RUO
Other Names: PVR (poliovirus receptor) homolog
Isotype: Rat IgG2a, κ