Biolegend, 132005, PE anti-mouse CD226 (DNAM-1) Antibody, 25μg

CD226 (DNAM-1) is constitutively expressed on native CD8+ cells and on some CD4+ T cells, macrophages and NK cells. CD226 is involved in NK and T cell mediated cytotoxicity against certain tumors. CD155 and CD112 are the ligands for CD226. This antibody (480.1) is reported by the developer to partially block the binding of mouse CD155.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Jurkat cells transfected with mouse CD226
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): Dardalhon V, et al. 2005. J. Immunol. 175:1558
Product Citations: He Y, et al. 2017. J Autoimmun. 10.1016/j.jaut.2017.04.001. PubMed Victorino F, et al. 2021. eLife. 0.416666666666667. PubMed
RRID: AB_1279173 (BioLegend Cat. No. 132006)
Structure: 65 kd glycoprotein of a member of Ig-superfamily containing 2 immunologlobulin-like domains.
Distribution: Expressed on majority of CD8+ and CD4+ T cells, NK cells, monocytes/macrophages and a subset of B cells. Reported to upregulated on Th1 cells, but downregulated in Th2 cells.
Function: Mediates cellular adhesion, involved in LFA-1 co-stimulatory signal for T cell differentiation and proliferation
Ligand/Receptor: CD112 and CD155
Cell Type: B cells, Macrophages, Monocytes, NK cells, T cells, Th1
Biology Area: Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Tahara Hanaoka S et al. 2006 Blood 107:1491 2. Shibuya K et al. 2003. J. Exp. Med. 198:1829
Gene ID: 225825
UniProt: View information about CD226 on UniProt.org
Clone: 480.1
Regulatory Status: RUO
Other Names: DNAM-1, PTA1(platelet and T cell activation antigen 1), TLISA1, LFA-1 associated Molecule PTA-1
Isotype: Rat IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.