Biolegend, 133303, PE anti-mouse Lineage Cocktail with Isotype Ctrl, 25tests

The mouse lineage panel has been designed to react with cells from the major hematopoietic cell lineages, such as T lymphocytes, B lymphocytes, monocytes/macrophages, granulocytes, NK cells, and erythrocytes. The PE Mouse Lineage Antibody Cocktail is designed for the flow cytometric identification of hematopoietic progenitors in mouse bone marrow. Components include anti-mouse CD3ε, clone 145-2C11; anti-mouse Ly-6G/Ly-6C, clone RB6-8C5; anti-mouse CD11b, clone M1/70; anti-mouse CD45R/B220, clone RA3-6B2; anti-mouse TER-119/Erythroid cells, clone Ter-119. PE Mouse Lineage Isotype Control Cocktail contains equivalent concentrations of isotype-matched negative control immunoglobulin. Materials provided: 1. PE anti-mCD3ε/ PE anti-mGr-1/ PE anti-mCD11b/ PE anti-mCD45R(B220)/ PE anti-mTer-119, 25 tests 2. PE Armenian hamster IgG/ PE Rat IgG2b/ PE Rat IgG2a isotype controls, 25 tests Materials not provided: Cell Staining Buffer (Cat. No. 420201)
25tests
Verified Reactivity: Mouse
Formulation: This two-reagent set contains PE conjugated antibodies and isotype controls in optimal concentration for flow cytometric analysis.
Preparation: The antibodies were purified by affinity chromatography, and conjugated with PE under optimal conditions.
Storage & Handling: It should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of these antibodies is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 20 µl per million cells or 20 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): Morris SJ, et al. 1997. Development. 124(10):1929-1939 Okada S, et al. 1992. Blood180 (12):3044-3050 Spangrude GJ, et al. 1988. Science 241 (4861):58-62 Spangrude GJ, et al. 1990. Exp Hematol. 18 (8):920-926 Tan SL, et al. 2006. J. Immunol. 176:2872. PubMed Ito R, et al. 2012. Exp Hematol. 40:953. PubMed.
Product Citations: Harsha Krovi S, et al. 2020. Nat Commun. 4.790277778. PubMed Omer-Javed A, et al. 2022. Cell. 185:2248. PubMed Lei F, et al. 2020. Proc Natl Acad Sci U S A. 117:23336. PubMed Antunes KH, et al. 2022. Front Immunol. 13:867022. PubMed Ito R, et al. 2012. Exp Hematol. 40:953. PubMed Rosu A, et al. 2021. Journal of Experimental Medicine. 218(3):. PubMed Verma A, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.26. PubMed Chen C, et al. 2020. Cell Rep. 2136:30. PubMed Chetty A, et al. 2021. Cell Host Microbe. 29:579. PubMed
RRID: AB_1595553 (BioLegend Cat. No. 133303)
Biology Area: Immunology
Gene ID: 104231
UniProt: View information about Lineage on UniProt.org
Clone: 145-2C11; RB6-8C5; RA3-6B2; Ter-119; M1/70;
Regulatory Status: RUO
Other Names: Lin-1
Isotype: Armenian Hamster IgG/Rat IgG2a/Rat IgG2b
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.