Biolegend, 133310, Pacific Blue™ anti-mouse Lineage Cocktail, 100tests

The mouse lineage panel has been designed to react with cells from the major hematopoietic cell lineages, such as T lymphocytes, B lymphocytes, monocytes/macrophages, granulocytes, NK cells, and erythrocytes. The Pacific Blue™ Mouse Lineage Antibody Cocktail is designed for the flow cytometric identification of hematopoietic progenitors in mouse bone marrow. Components include anti-mouse CD3, clone 17A2; anti-mouse Ly-6G/Ly-6C, clone RB6-8C5; anti-mouse CD11b, clone M1/70; anti-mouse CD45R/B220, clone RA3-6B2; anti-mouse TER-119/Erythroid cells, clone Ter-119. Materials provided: 1. Pacific Blue™ anti-mCD3/ Pacific Blue™ anti-mLy-6G(Ly-6C)/ Pacific Blue™ anti-mCD11b/ Pacific Blue™ anti-mCD45R(B220)/ Pacific Blue™ anti-mTer-119 Materials not provided: 1. Cell Staining Buffer (Cat. No. 420201) 2. Isotype Controls
100tests
Verified Reactivity: Mouse
Host Species: Rat
Formulation: This set contains Pacific Blue™ conjugated antibodies in an optimal concentration for flow cytometric analysis.
Preparation: The antibodies are purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of these antibodies is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 20 µl per million cells or 20 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Violet Laser (405 nm)
Product Citations: Tikka C, et al. 2023. Front Immunol. 14:1026368. PubMed Sambandam A, et al. 2023. Heliyon. 9:e14238. PubMed Luo L, et al. 2023. Theranostics. 13:2072. PubMed Zhou J, et al. 2023. Nutrients. 15: . PubMed Li DD, et al. 2022. Cell Host Microbe. 30:530. PubMed Labuhn M, et al. 2020. Cancer Cell. 36(2):123-138. PubMed He Y, et al. 2021. Infect Immun. 89:. PubMed Sharma GP, et al. 2021. PLoS One. 16:e0259042. PubMed Snyder LM, et al. 2021. PLoS Pathog. 17:e1009970. PubMed Dráberová L, et al. 2020. Front Immunol. 10:3068. PubMed Simon M, et al. 2019. Cell Metab. 29:871. PubMed Lukowski SW et al. 2019. Cell Rep. 27(9):2748-2758 . PubMed Chun E, et al. 2020. Immunity. 51(5):871-884.e6.. PubMed Trompette A, et al. 2022. Mucosal Immunol. :. PubMed Malik A et al. 2018. Immunity. 49(3):515-530 . PubMed Capitano ML, et al. 2019. J Clin Invest. 130:2555. PubMed Frech S, et al. 2021. J Invest Dermatol. . PubMed Mistry JJ, et al. 2021. Nat Commun. 12:7130. PubMed Fang H, et al. 2020. Nat Commun. 4.661805556. PubMed van Gastel N, et al. 2020. Cell Metabolism. 32(3):391-403.e6. PubMed Goldstein JM et al. 2019. Cell reports. 27(4):1254-1264 . PubMed Wang T et al. 2018. Immunity. 49(3):504-514 . PubMed Capitano ML, et al. 2021. J Clin Invest. 131:. PubMed Kenty JH, et al. 2021. STAR Protocols. 2(3):100675. PubMed Aurélien Trompette et al. 2018. Immunity. 48(5):992-1005 . PubMed
RRID: AB_11150779 (BioLegend Cat. No. 133310)
Distribution: T cells, B cells, monocytes/macrophages, granulocytes, NK cells, and erythrocytes
Cell Type: B cells, Erythrocytes, Granulocytes, Macrophages, Monocytes, NK cells, T cells
Biology Area: Immunology
Antigen References: 1. Morrison SJ, et al. 1997. Development 124:1929. 2. Okada S, et al. 1992. Blood 80:3044. 3. Spangrude GJ, et al. 1988. Science 241:58. 4. Spangrude GJ, et al. 1990. Exp. Hematol. 18:920. 5. Tan SL, et al. 2006. J. Immunol. 176:2872.
Gene ID: 104231
UniProt: View information about Lineage on UniProt.org
Clone: 17A2; RB6-8C5; RA3-6B2; Ter-119; M1/70;
Regulatory Status: RUO
Other Names: Lin-1
Isotype: Rat IgG2a/Rat IgG2b