Biolegend, 133403, PE anti-mouse CD267 (TACI) Antibody, 50μg

Transmembrane Activator and CAML Interactor (TACI) is a tumor necrosis factor receptor superfamily (TNFSF) member. It is a type III transmembrane protein sharing a high degree of homology with B cell maturation antigen (BCMA) among TNFSF members. It binds to the ligands BAFF (BLyS) and APRIL. CD267 appears to be particularly important for the regulation of B cell survival and maturation in the spleen. It is a major mediator of BAFF-dependent costimulatory responses in peripheral B cells. Signaling through TACI may serve to down-regulate B cell activity as well. The actual role for TACI in the mouse has still not been fully determined.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Rat RBL transfected cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per 106 cells in 100 µl. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): Siegel RM, et al. 2001. Nat. Immunol. 2:577. Von Bulow GU, et al. 1997. Science 278:138. Yan M, et al. 2001. Nat. Immunol. 2:638. Diaz-de-Durana Y, et al. 2006. Blood 107:594. Moir S, et al. 2012. Blood. 120:4850. PubMed.
Product Citations: Agrafiotis A, et al. 2023. iScience. 26:106055. PubMed Pioli KT, et al. 2023. iScience. 26:106223. PubMed Chappaz S, et al. 2021. Cell Reports. 36(3):109430. PubMed Pack AD, et al. 2021. Cell Reports. 36:109586. PubMed Zhu H, et al. 2019. Nat Commun. 10:1084. PubMed Werner A, et al. 2021. iScience. 24:103076. PubMed Moir S, et al. 2012. Blood. 120:4850. PubMed
RRID: AB_2203542 (BioLegend Cat. No. 133403) AB_2240584 (BioLegend Cat. No. 133404)
Structure: TACI/CD267 is a type III transmembrane protein belonging to TNF receptor superfamily (TNFSF). Among TNFSF members, TACI shares a high degree of homology with B cell maturation antigen (BCMA). It binds to the ligands BAFF (BLyS) and APRIL.
Distribution: CD267 is expressed predominantly by splenic transitional type 2 and marginal zone B cells, as well as activated B cells. But it is notably absent from germinal center B cells.
Function: CD267 appears to be particularly important for the regulation of B cell survival and maturation in the spleen. It is a major mediator of BAFF-dependent costimulatory responses in peripheral B cells. Signaling through TACI may serve to down-regulate B cell activity.
Ligand/Receptor: BAFF (Blys/TALL-1/THANK) and APRIL
Cell Type: B cells
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Ng LG, et al. 2004. J. Immunology. 173:807.
Gene ID: 57916
UniProt: View information about CD267 on UniProt.org
Clone: 8F10
Regulatory Status: RUO
Other Names: TACI
Isotype: Rat IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.