Biolegend, 133615, Brilliant Violet 421™ anti-mouse CD226 (DNAM-1) Antibody, 50μg

Mouse CD226 (DNAM-1) is a 65 kd glycoprotein of a member of Ig super-family adhesion molecule containing 2 immunoglobulin-like domains. It is associated with leukocyte function associated antigen-1 (LFA-1) involving in TCR-mediated signal transduction for T cell proliferation and differentiation. CD226 is also involved in NK cell and T cell mediated cytotoxicity against certain tumors. CD226 (DNAM-1) is constitutively expressed on naïve CD8 + and CD4 + T cells, macrophages, and NK cells. It was reported that CD226 expression is up-regulated on Th1 cells, but down-regulated on Th2 and Th0 cells. CD155 and CD112 are the ligands for CD226.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse DNAM-1 transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application References(PubMed link indicates BioLegend citation): Dardalhon V, et al. 2005. J. Immunol. 175:1558 Shirakawa J, et al. 2006. International Immunology 18(6):951-957
Product Citations: Weulersse M, et al. 2020. Immunity. 53(4):824-839.e10. PubMed
RRID: AB_2715977 (BioLegend Cat. No. 133615)
Structure: 65 kd glycoprotein of a member of Ig super-family containing 2 immunoglobulin-like domains.
Distribution: Expressed on majority of CD8+ and CD4+ T cells, NK cells, monocytes/macrophages and a subset of B cells. Reported to up-regulated on Th1 cells, but down-regulated in Th2 cells.
Function: Mediate cellular adhesion, involved in LFA-1 co-stimulatory signal for T cell differentiation and proliferation.
Ligand/Receptor: CD112 and CD155
Cell Type: B cells, Macrophages, Monocytes, NK cells, T cells, Th1
Biology Area: Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1 Tahara Hanaoka S, et al. 2006. Blood. 107:1491 2 Shibuya K et al. 2003. J. Exp. Med. 198:1829.
Gene ID: 225825
UniProt: View information about CD226 on UniProt.org
Clone: TX42.1
Regulatory Status: RUO
Other Names: DNAM-1, PTA1 (Platelet and T cell activation antigen 1), TLISA1, LFA-1 associated molecule PTA-1
Isotype: Rat IgG2a, κ
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.