Biolegend, 134006, Alexa Fluor® 647 anti-mouse CD366 (Tim-3) Antibody, 100μg

CD366 (Tim-3) is a transmembrane protein also known as T cell immunoglobulin and mucin domain containing protein-3. Tim-3 is expressed at high levels on Th1 lymphocytes and CD11b + macrophages. Tim-3 has also been shown to exist as a soluble protein. Cells expressing Tim-3 are present at high levels in the CNS of animals at the onset of experimental autoimmune encephalomyelitis (EAE), a disease mediated by lymphocytes secreting Th1-like cytokines. Tim-3 has been proposed to inhibit Th1-mediated immune responses and promote immunological tolerance.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: mTim-3 protein/Freund adjuvant
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: Clone B8.2C12 only binds to the BALB/c allele of Tim-3.
Application References(PubMed link indicates BioLegend citation): del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
Product Citations: Tang M, et al. 2023. Sci Adv. 9:eade6624. PubMed Mizukami S, et al. 2015. Proc Natl Acad Sci U S A. 112:1809. PubMed Milner JJ, et al. 2020. Immunity. 52(5):808-824.e7. PubMed Palazon A, et al. 2017. Cancer Cell. . 10.1016/j.ccell.2017.10.003. PubMed Ersching J, et al. 2016. PLoS Pathog. 12: 1005698. PubMed
RRID: AB_1626179 (BioLegend Cat. No. 134005) AB_1626175 (BioLegend Cat. No. 134006)
Structure: Transmembrane protein containing immunoglobulin domain and mucin-like domain. It can exist as a soluble form lacking mucin and transmembrane domains.
Distribution: Expressed on activated Th1 lymphocytes, CD11b+ macrophages, and dendritic cells.
Function: Play a role in regulating macrophage activation, T cell apoptosis and immune tolerance.
Ligand/Receptor: Galectin -9
Cell Type: Dendritic cells, Macrophages, Th1
Biology Area: Immunology, Inhibitory Molecules
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Antigen References: 1. Sabatos CA, et al. 2003. Nat. Immunol. 4:1102 2. Kuchroo VK, et al. 2003. Nat. Rev. Immunol. 3:454 3. Mooney L, et al. 2002. Nature. 415:536 4. Rodriguez-Manzanet R, et al. 2009. Immunol. Rev. 229(1):259
Gene ID: 171285
UniProt: View information about CD366 on UniProt.org
Clone: B8.2C12
Regulatory Status: RUO
Other Names: T cell immunoglobulin and mucin domain containing protein 3, hepatitis virus cellular receptor 2, CD366
Isotype: Rat IgG1, κ