Biolegend, 134202, Purified anti-mouse CD178 (FasL) Antibody, 100μg

CD178 is a 40 kD member of the TNF superfamily also known as Fas ligand or FasL. Cell surface CD178 is expressed on activated T cells and in testis and eye. CD178 is upregulated in activated T cells upon TCR re-engagement and has been shown to induce T cell death. CD178 expression in the eye and testis has been shown to participate in immune privilege at these sites. CD178 can be cleaved from the surface by metalloproteases, and "soluble" CD178 may block the activities of membrane-bound CD178. CD178 binds to CD95 (Fas) to induce apoptotic cell death implicated in the maintenance of peripheral tolerance. CD178/CD95 interactions have also been implicated in the proliferation of CD8+ cells and neutrophil extravasation, chemotaxis, and survival.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant mouse FasL
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone A19116F does not affect the binding of clone MFL3 on target cells.
RRID: AB_2936669 (BioLegend Cat. No. 134202)
Structure: TNF superfamily, 40 kD
Distribution: Activated T cells, spleen, testis, eye
Function: Induces apoptosis
Ligand/Receptor: Fas (CD95)
Cell Type: T cells, Tregs
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Biology, Immunology, Neuroscience
Molecular Family: CD Molecules
Antigen References: Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. Nagata S. 1999. Annu Rev Genet. 33:29-55. Takahashi T, et al. 1994. Cell. 76:969-76. Hill LL, et al. 1999. Science. 285:898-900.
Gene ID: 14103
UniProt: View information about CD178 on UniProt.org
Clone: A19116F
Regulatory Status: RUO
Isotype: Mouse IgG2a, κ