Biolegend, 134605, PE anti-mouse Ly108 Antibody, 25μg

Mouse Ly108, also known as SLAMF6 and NTB-A (NK cell, T cell, B cell antigen), is one of the members in The Signaling Lymphocytic Activation Molecule (SLAM) family of immune receptors. It is expressed on T cells, B cells, macrophages, dendritic cells, NK cells, and granulocytes. Homophilic interaction of Ly108 is involved in augmenting cytotoxicity of NK cells. Ly108 has been shown to function on NK cells by augmenting cytotoxicity. It was reported that Ly108 plays an important role in CD4 + T cell responses and innate immunity to bacteria and parasites. In a mouse with a targeted disruption of the Ly108 gene, CD4 + T cells and innate responses are defective. SLAM family of receptors has been implicated in the pathophysiology of autoimmunity. For instance, Ly108 is strongly linked to lupus susceptibility in mice. Ly108 may censor self-reactive B cells as a potential regulator of tolerance checkpoints, safeguarding against autoimmunity. Therefore, Ly108 serves as a regulator of both innate and adaptive immune responses.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Thymocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): Fu GF, et al. 2012. Int Immunol. 24:197. PubMed.
Product Citations: Li X, et al. 2023. J Clin Invest. 133:. PubMed del Rio ML, et al. 2023. Front Immunol. 14:1113858. PubMed Wight AE, et al. 2022. Proc Natl Acad Sci U S A. 119:e2200757119. PubMed Fitzgerald B, et al. 2021. Cell Rep Methods. 1:. PubMed Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed Siddiqui I et al. 2019. Immunity. 50(1):195-211 . PubMed Chen Z et al. 2019. Immunity. 51(5):840-855 . PubMed Beltra JC, et al. 2020. Immunity. 52(5):825-841. PubMed De Ponte Conti B, et al. 2021. Elife. 10:. PubMed Blanchard L, et al. 2022. STAR Protoc. 3:101444. PubMed Chen Z, et al. 2021. Cell. 184(5):1262-1280.e22. PubMed Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed Fu G, et al. 2012. Int Immunol. 24:197. PubMed
RRID: AB_1659258 (BioLegend Cat. No. 134605) AB_2188095 (BioLegend Cat. No. 134606)
Structure: A glycoprotein of the signaling lymphocytic activation molecule family of cell surface receptor. It is a novel member of the CD2 subfamily of the Ig superfamily.
Distribution: Expressed on T cells, B cells, macrophages, dendritic cells, NK cells, and granulocytes.
Function: Play an important role in CD4+ T cell responses, innate immunity to bacteria and parasites, and NK cell function.
Ligand/Receptor: Ly108
Cell Type: B cells, Dendritic cells, Granulocytes, Macrophages, NK cells, T cells
Biology Area: Immunology, Innate Immunity
Antigen References: 1. Howie D, et al. 2005. J. Immunology. 174 (10):5931 2 Kumar KR, et al. 2006. Science. 312(5780):1665 3. Zhong MC, et al. 2008. J. Biol. Chem. 283 (28):19255 4. Peck SR et al. 2000. Immunogenetics. 52:63
Gene ID: 30925
UniProt: View information about Ly108 on UniProt.org
Clone: 330-AJ
Regulatory Status: RUO
Other Names: SLAM family receptor, SLAMF6, TCOM, SF2000, KALI, SF-3, NTBA, NTB-A, NK-T-B antigen
Isotype: Mouse IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.