Biolegend, 134809, APC anti-mouse CD272 (BTLA) Antibody, 25μg

B and T lymphocyte attenuator (BTLA) is an Ig superfamily coinhibitory receptor with structural similarity to programmed cell death 1 (PD-1) and CTLA-4. BTLA is expressed on B cells, T cells, macrophages, dendritic cells, NKT cells, and NK cells. Engagement of BTLA by its ligand Herpes Virus Entry Mediator (HVEM) is critical for negatively regulating immune response. The absence of BTLA with HVEM inhibitory interactions leads to increased experimental autoimmune encephalomyelitis severity, enhanced rejection of partially mismatched allografts, an increased CD8 + memory T cell population, increased severity of colitis, reduced effectiveness of T regulatory cells. BTLA takes an important role in the induction of peripheral tolerance of both CD4 + and CD8 + T cells in vivo. Tolerant T cells have significant up-regulated expression of BTLA compared with effector and naïve T cells. BTLA may cooperate with CTLA-4 and PD-1 to control T cell tolerance and autoimmunity. It was reported that BTLA may regulate T cell function by binding to B7-H4. But further studies are needed to confirm. The existence of three distinct BTLA alleles was reported. The BTLA antibody reacts with mouse BTLA from both BALb/c and C57BL/6 strains.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: C57BL/6 BTLA Ig domain protein in CFA
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application References(PubMed link indicates BioLegend citation): Hurchla MA, et al. 2005. J. Immunol. 174:3377
RRID: AB_2067835 (BioLegend Cat. No. 134809) AB_2067834 (BioLegend Cat. No. 134810)
Structure: An Ig superfamily coinhitory receptor with structural similarity to programmed cell death 1 (PD-1) and CTLA-4.
Distribution: BTLA is expressed on a wide number of lymphocytes in mice. It is most highly expressed on B cells, followed by CD4+T cells, lower express on CD8+ T cells, macrophages, dendritic cells, NKT cells, and NK cells.
Function: BTLA functions as a negative regulator of T cell activation and proliferation. It attenuates B cell proliferation upon associating with its known ligand, Herpes Virus Entry Mediator (HVEM).
Ligand/Receptor: Herpes Virus Entry Mediator (HVEM)
Cell Type: Lymphocytes, B cells, T cells, Dendritic cells, NKT cells
Biology Area: Immunology, Inhibitory Molecules
Molecular Family: CD Molecules
Antigen References: 1. Liu X, et al. 2009. J. Immunol. 182:45162. Miller ML, et al. 2009. J. Immunol. 183:323. Sun Y, et al. 2009. J. Immunol. 183:19464. Vendel AC, et al. 2009. J. Immunol. 182:15095. Watanabe N, et al. 2003. Nat. Immunol. 4(7):6706. Sedy JR, et al. 2005. Nat Immunol. 6(1):90
Gene ID: 208154
UniProt: View information about CD272 on UniProt.org
Clone: 8F4
Regulatory Status: RUO
Other Names: BTLA, B and T lymphocyte attenuator
Isotype: Mouse IgG1, κ