Biolegend, 135005, Biotin anti-mouse CD127 (IL-7Rα) Antibody, 50μg

CD127 is a 60-90 kD type I transmembrane glycoprotein also known as IL-7 receptor α chain or IL-7Rα. It forms a heterodimer with the common γ chain (γc or CD132) which is shared with the receptors for IL-2, IL-4, IL-9, IL-13, IL-15, and IL-21. CD127 is expressed on immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, and bone marrow stromal cells. CD127 has been reported to be an useful marker for identifying memory and effector T cells. The ligation of IL-7 with its receptor is important for stimulation of mature and immature T cells as well as immature B cells proliferation and development.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: IL-7Ra-IgG1 fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone A7R34 is able to completely block the binding of clone S18006K to IL-7Rα. It does not block SB/199.
Application References(PubMed link indicates BioLegend citation): Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125. Hashi H, et al. 2001. J. Immunol. 166:3702. Taylor R, et al. 2007. J. Immunol. 178:5659. Mazzon C, et al. 2011. Blood. 118:2733. PubMed Jin J, et al. 2011. J. Immunol. doi:10.4049/jimmunol.1001238. PubMed
Product Citations: Bouchareychas L, et al. 2021. iScience. 24(8):102847. PubMed Florentin J, et al. 2022. Front Immunol. 13:882484. PubMed Fuster JJ, et al. 2020. Cell Rep. 33:108326. PubMed Lu SX, et al. 2021. Cell. . PubMed Mann M, et al. 2018. Cell Rep. 25:2992. PubMed Dave K et al. 2017. eLife. 6 pii: e23382. PubMed Liu YY, et al. 2020. Chin Med J (Engl). 133:1688. PubMed Kim SP, et al. 2021. Cell Reports. 36(9):109626. PubMed Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed Knolle MD et al. 2018. Frontiers in immunology. 1.925 . PubMed Yankova E, et al. 2021. Nature. 593:597. PubMed Murakami K, et al. 2021. Cell Reports. 34(1):108579. PubMed Bouchareychas L, et al. 2020. Cell Reports. 32(2):107881. PubMed Bagadia P, et al. 2019. Nat Immunol. 20:1174. PubMed Vasamsetti SB, et al. 2018. Immunity. 49:93. PubMed Michel A, et al. 2013. J Immunol. 90:5534. PubMed Shimba A et al. 2018. Immunity. 48(2):286-298 . PubMed Yoshida H, et al. 2019. Cell. 176:897. PubMed Riedel SS, et al. 2021. Molecular Cell. 81(11):2332-2348.e9. PubMed Wang J, et al. 2020. Cell Stem Cell. 27(1):81-97. PubMed Li ZG, et al. 2020. Chin Med J (Engl). 1943:133. PubMed
RRID: AB_1953262 (BioLegend Cat. No. 135005) AB_2126118 (BioLegend Cat. No. 135006)
Structure: Type I transmembrane glycoprotein, associate with CD132, 60-90 kD
Distribution: Immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, bone marrow stromal cells
Function: T cell and immature B cell proliferation and development
Ligand/Receptor: IL-7
Cell Type: B cells, T cells, Thymocytes
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125. 2. Okuno Y, et al. 2001. P. Natl. Acad. Sci. USA 99:6246. 3. Pillai M, et al. 2004. Leukemia Lymphoma 45:2403.
Gene ID: 16197
UniProt: View information about CD127 on UniProt.org
Clone: A7R34
Regulatory Status: RUO
Other Names: IL-7 receptor α chain, IL-7Rα
Isotype: Rat IgG2a, κ
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.