Biolegend, 135020, Alexa Fluor® 647 anti-mouse CD127 (IL-7Rα) Antibody, 100μg

CD127 is a 60-90 kD type I transmembrane glycoprotein also known as IL-7 receptor α chain or IL-7Rα. It forms a heterodimer with the common γ chain (γc or CD132) which is shared with the receptors for IL-2, IL-4, IL-9, IL-13, IL-15, and IL-21. CD127 is expressed on immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, and bone marrow stromal cells. CD127 has been reported to be an useful marker for identifying memory and effector T cells. The ligation of IL-7 with its receptor is important for stimulation of mature and immature T cells as well as immature B cells proliferation and development.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: IL-7Ra-IgG1 fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: Clone A7R34 is able to completely block the binding of clone S18006K to IL-7Rα. It does not block SB/199.
Application References(PubMed link indicates BioLegend citation): Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125. Hashi H, et al. 2001. J. Immunol. 166:3702. Taylor R, et al. 2007. J. Immunol. 178:5659. Mazzon C, et al. 2011. Blood. 118:2733. PubMed Jin J, et al. 2011. J. Immunol. doi:10.4049/jimmunol.1001238. PubMed
Product Citations: Doisne J, et al. 2015. J Immunol. 195: 3937 - 3945.. PubMed Giannou AD, et al. 2023. Immunity. 56:125. PubMed Zhu Y, et al. 2021. EMBO J. 40:e105320. PubMed Balzano M et al. 2019. Cell reports. 26(12):3257-3271 . PubMed Shen B, et al. 2021. Nature. 591:438. PubMed Corrado M, et al. 2020. Cell Metab. 32:981. PubMed
RRID: AB_1937210 (BioLegend Cat. No. 135019) AB_1937209 (BioLegend Cat. No. 135020)
Structure: Type I transmembrane glycoprotein, associate with CD132, 60-90 kD
Distribution: Immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, bone marrow stromal cells
Function: T cell and immature B cell proliferation and development
Ligand/Receptor: IL-7
Cell Type: B cells, T cells, Thymocytes
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125. 2. Okuno Y, et al. 2001. P. Natl. Acad. Sci. USA 99:6246. 3. Pillai M, et al. 2004. Leukemia Lymphoma 45:2403.
Gene ID: 16197
UniProt: View information about CD127 on UniProt.org
Clone: A7R34
Regulatory Status: RUO
Other Names: IL-7 receptor α chain, IL-7Rα
Isotype: Rat IgG2a, κ