Biolegend, 135122, Brilliant Violet 605™ anti-mouse CD117 (c-kit) Antibody, 500microl

CD117 is a 145 kD immunoglobulin superfamily member, also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with its ligand is critical for the development of hematopoietic stem cells.
500microl
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Murine IL-3 dependent mast cells
Formulation: µl sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions.
Concentration: µg sizes: 0.2 mg/mLµL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µl sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Injection of ACK2 in mice of NOD genetic background is not recommended as mice develop immediate anaphylaxis, resulting in animal death. Treatment is possible with co-injection of Benadryl.3 ACK2 has been reported to block c-Kit function. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 135103). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 135114) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Application References(PubMed link indicates BioLegend citation): Yoshinaga K, et al. 1991. Development 113:689. Broudy VC, et al. 1996. Blood 88:75. Louvet C, et al. 2008. Proc. Natl. Acad. Sci. USA 105:18895.
Product Citations: Oetjen LK et al. 2017. Cell. 171(1):217-228 . PubMed Chen S, et al. 2022. Cancer Discov. :. PubMed Benhamou D, et al. 2018. Front Immunol. 2.260416667. PubMed Inoue D, et al. 2021. Nat Genet. 53:707. PubMed Yoshimi A, et al. 2019. Nature. 574:273. PubMed Montel-Hagen A, et al. 2020. Cell Rep. 33:108320. PubMed Green DP, et al. 2019. Neuron. 101:412. PubMed Lopez DA, et al. 2022. Cell Rep. 41:111677. PubMed Wang F, et al. 2021. Cell. 184(2):422-440.e17. PubMed Gentek R, et al. 2018. Immunity. 48:1160. PubMed Filtjens J, et al. 2021. Nat Commun. 12:2936. PubMed
RRID: AB_2562040 (BioLegend Cat. No. 135121) AB_2650925 (BioLegend Cat. No. 135120) AB_2562042 (BioLegend Cat. No. 135122)
Structure: Ig superfamily, 145 kD
Distribution: Hematopoietic stem cells, AML, mast cells, oocytes, interstitial theca cells, leydig cells
Function: Growth factor receptor, tyrosine kinase
Ligand/Receptor: Stem Cell Factor (SCF)
Cell Type: Embryonic Stem Cells, Mesenchymal Stem Cells
Biology Area: Immunology, Stem Cells
Molecular Family: CD Molecules
Antigen References: 1. Barclay A et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Galli SJ. et al. 1994. Adv. Immunol. 55:1. 3. Ikuta K et al. 1992. Annu. Rev. Immunol. 10:759. 4. Besmer P et al. 1986. Nature 320:415. 5. Witte ON. et al. 1990. Cell 63:5.
Gene ID: 16590
UniProt: View information about CD117 on UniProt.org
Clone: ACK2
Regulatory Status: RUO
Other Names: c-KIT, Stem Cell Factor Receptor (SCFR)
Isotype: Rat IgG2b, κ