Biolegend, 135224, APC/Cyanine7 anti-mouse CD279 (PD-1) Antibody, 100μg

CD279, also known as programmed death-1 (PD-1), is a 50-55 kD glycoprotein belonging to the CD28 family of the Ig superfamily. PD-1 is expressed on activated splenic T and B cells and thymocytes. It is induced on activated myeloid cells as well. PD-1 is involved in lymphocyte clonal selection and peripheral tolerance through binding its ligands, B7-H1 (PD-L1) and B7-DC (PD-L2). It has been reported that PD-1 and PD-L1 interactions are critical to positive selection and play a role in shaping the T cell repertoire. PD-L1 negative costimulation is essential for prolonged survival of intratesticular islet allografts.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: PD-1 cDNA followed by PD-1-Ig fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with APC/Cyanine7 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue3, in vivo blocking of PD-1 binding to its ligands2,3, and spatial biology (IBEX)5,6.
Application References(PubMed link indicates BioLegend citation): Good-Jacobson KL, et al. 2010. Nat. Immunol. 11:535. (FC) PubMed Lázár-Molnár E, et al. 2008. Proc. Natl. Acad. Sci. USA 105:2658. (Block) Liang SC, et al. 2003. Eur. J. Immunol. 33:2706. (FC, IHC, Block) Tobias J, et al. 2020. Front Immunol. 11:895 (FC, ELISA) PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Zander R, et al. 2022. Elife. 11:. PubMed Battistello E, et al. 2023. Mol Cell. 83:1216. PubMed Corria-Osorio J, et al. 2023. Nat Immunol. 24:869. PubMed Pandit M, et al. 2023. Nat Commun. 14:2593. PubMed Eberhardt F, et al. 2023. Int J Mol Med. 52:. PubMed Zander R, et al. 2022. Immunity. 55:475. PubMed Hailemichael Y, et al. 2022. Cancer Cell. 40:509. PubMed Xu W et al. 2019. Immunity. 50(4):1054-1068 . PubMed Ersching J et al. 2017. Immunity. 46(6):1045-1058 . PubMed Greenberg J, et al. 2022. JCI Insight. Online ahead of print. PubMed Shan M et al. 2018. Immunity. 49(4):709-724 . PubMed Snell LM, et al. 2018. Immunity. 49:678. PubMed Li J, et al. 2018. Immunity. 49:178. PubMed Hildebrand KM, et al. 2021. PLoS One. 16:e0253864. PubMed Mender I, et al. 2020. Cancer Cell. 38(3):400-411.e6. PubMed Leclerc M, et al. 2019. Nat Commun. 10:3345. PubMed Zhou J, et al. 2022. Int J Oncol. 61: . PubMed Andersen TK, et al. 2019. NPJ Vaccines. 4:9. PubMed Fedele C, et al. 2021. J Exp Med. 218: . PubMed Malenica I, et al. 2021. Nat Commun. 12:5209. PubMed Mesin L, et al. 2020. Cell. 180(1):92-106.e11.. PubMed Song X, et al. 2022. Transl Oncol. 15:101306. PubMed
RRID: AB_2563522 (BioLegend Cat. No. 135223) AB_2563523 (BioLegend Cat. No. 135224)
Structure: A 50-55 kD glycoprotein belonging to the CD28 family of the Ig superfamily.
Distribution: Induced on splenic T and B lymphocytes, thymocytes, and myeloid cells after stimulation.
Function: Involved in lymphocyte clonal selection and peripheral tolerance, prolonged survival of allografts.
Ligand/Receptor: B7-H1 (PD-L1) and B7-DC (PD-L2)
Cell Type: B cells, T cells
Biology Area: Cancer Biomarkers, Immunology, Inhibitory Molecules
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Antigen References: 1. Nishimura H, et al. 2001. Science 291:319 2. Agata Y, et al. 1996. Int. Immunol. 8:765 3. Liang SC, et al. 2003. Eur. J. Immunol. 33:2706 4. Barber DL, et al. 2006. Nature 439:682 5. Keir ME, et al. 2005. J. Immunol. 175:7372 6. Koehn BH. et al. 2008. J Immunol. 181:5313
Gene ID: 18566
UniProt: View information about CD279 on UniProt.org
Clone: 29F.1A12
Regulatory Status: RUO
Other Names: PD-1, Programmed Death-1, PDCD1
Isotype: Rat IgG2a, κ