Biolegend, 135502, Purified anti-mouse CD115 (CSF-1R) Antibody, 500μg

CSF-1R, also known as CD115 and M-CSFR, is a single-pass type I membrane protein and member of the platelet-derived growth factor receptor family. This c-fms (Fms proto-oncogene) gene product's natural ligands include M-CSF and IL-34. Structural studies of CD115 have described an Ig-like extracellular domain, a transmembrane domain, an intracellular juxtamembrane domain, a split tyrosine kinase domain, and a C-terminal tail receptor. Receptor activation induces homodimerization in addition to phosphorylation and ubiquitination of intracellular residues. CD115 directly influences tissue macrophage and osteoclast differentiation and proliferation. It is expressed on monocytes/macrophages, peritoneal exudate cells, plasmacytoid and conventional dendritic cells, and osteoclasts.
500μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality testedBlock - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: blocking of ligand binding1. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays.It has been reported that CD115 can be rapidly internalized, especially when samples are exposed to room temperature. Approximate 33% decrease in CD115 expression has been observed between 0 and 4 hours after sample collection, while overnight incubation of the cells results in complete CD115 downregulation. Pre-treatment with EDTA and low temperatures (2 to 8°C) helps in maintaining surface expression of CD1154.
Application References(PubMed link indicates BioLegend citation): Sudo T, et al. 1995. Oncogene 11:2469. Murayama T, et al. 1999. Circulation 99:1740. Jaeger BN, et al. 2012. J. Exp. Med. 209:565. PubMed Breslin WL, et al. 2013. J Immunol Methods. 390(1-2):1 PubMed Dong L, et al. 2016. Nature. 539:304–308. PubMed
Product Citations: Wolf Y, et al. 2017. J Exp Med. 214:905. PubMed Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed Valet C, et al. 2022. J Clin Invest. 132: . PubMed Moore AR, et al. 2022. Cell Rep. 41:111651. PubMed Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed Donado CA, et al. 2020. Cell Reports. 31(1):107466. PubMed Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed Kapellos TS, et al. 2017. Mediators Inflamm. 2017:4315412. PubMed Inclan-Rico JM, et al. 2020. PLoS Pathog. 16:e1008579. PubMed Zhu YP et al. 2018. Cell reports. 24(9):2329-2341 . PubMed Rogers P, Driessnack M, and Schwartz E. 2017. PLoS One. 10.1371/journal.pone.0181985. PubMed Jaeger B, et al. 2012. J Exp Med. 209:565. PubMed Winter C, et al. 2018. Cell Metab. 28:175. PubMed Kikuchi K, et al. 2018. J Immunol. 201:635. PubMed Leist SR, et al. 2019. PLoS One. 14:e0220126. PubMed Regan–Komito D, et al. 2017. Front Immunol. 1.459027778. PubMed Seehus CR, et al. 2017. Nat Commun. 8:1900. PubMed
RRID: AB_1937292 (BioLegend Cat. No. 135501) AB_1937293 (BioLegend Cat. No. 135502)
Structure: Ig superfamily, 145 kD
Distribution: Monocytes/macrophages, peritoneal exudate cells, plasmacytoid and conventional dendritic cells, osteoclasts
Function: Growth factor receptor, tyrosine kinase
Ligand/Receptor: Macrophage colony stimulating factor (M-CSF), IL-34
Cell Type: Dendritic cells, Macrophages, Monocytes, Osteoclasts
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: Sudo T, et al. 1995 Oncogene 11:2469. Murayama T, et al. 1999 Circulation 99:1740. Goswami S, et al. 2005 Cancer Res. 65:5278. Yu W, et al. 2008 J. Leuko. Biol. 84:852.
Gene ID: 12978
UniProt: View information about CD115 on UniProt.org
Clone: AFS98
Regulatory Status: RUO
Other Names: CSF-1R, M-CSFR, c-fms
Isotype: Rat IgG2a, κ
Q: Why do I have a weak CD115 staining?
A: It has been reported that CD115 can be rapidly internalized, especially when samples are exposed to room temperature. Approximate 33% decrease in CD115 expression has been observed between 0 and 4 hours after sample collection, while overnight incubation of the cells results in complete CD115 downregulation.  Pre-treatment with EDTA and low temperatures (2 - 8°C) helps in maintaining surface expression of CD115. In addition, brief fixation of the cells with Fixation Buffer (Cat. No. 420801) for 10 minutes will block CD115 internalization.