Biolegend, 135513, Brilliant Violet 421™ anti-mouse CD115 (CSF-1R) Antibody, 50μg

CSF-1R, also known as CD115 and M-CSFR, is a single-pass type I membrane protein and member of the platelet-derived growth factor receptor family. This c-fms (Fms proto-oncogene) gene product's natural ligands include M-CSF and IL-34. Structural studies of CD115 have described an Ig-like extracellular domain, a transmembrane domain, an intracellular juxtamembrane domain, a split tyrosine kinase domain, and a C-terminal tail receptor. Receptor activation induces homodimerization in addition to phosphorylation and ubiquitination of intracellular residues. CD115 directly influences tissue macrophage and osteoclast differentiation and proliferation. It is expressed on monocytes/macrophages, peritoneal exudate cells, plasmacytoid and conventional dendritic cells, and osteoclasts.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Additional reported applications (for the relevant formats) include: blocking of ligand binding1. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays.It has been reported that CD115 can be rapidly internalized, especially when samples are exposed to room temperature. Approximate 33% decrease in CD115 expression has been observed between 0 and 4 hours after sample collection, while overnight incubation of the cells results in complete CD115 downregulation. Pre-treatment with EDTA and low temperatures (2 to 8°C) helps in maintaining surface expression of CD1154.
Application References(PubMed link indicates BioLegend citation): Sudo T, et al. 1995. Oncogene 11:2469. Murayama T, et al. 1999. Circulation 99:1740. Jaeger BN, et al. 2012. J. Exp. Med. 209:565. PubMed Breslin WL, et al. 2013. J Immunol Methods. 390(1-2):1 PubMed Dong L, et al. 2016. Nature. 539:304–308. PubMed
Product Citations: Duan Y, et al. 2019. Circ Res. 125:969. PubMed Hiyoshi H, et al. 2022. Cell Host Microbe. 30:163. PubMed Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed Gabriely G, et al. 2021. iScience. 24:103347. PubMed Götz P, et al. 2022. Int J Mol Sci. 23:. PubMed Bowers E, et al. 2018. Nat Med. 24:95. PubMed Tuttle KD, et al. 2020. Cell Rep. 33:108407. PubMed Hofbauer D, et al. 2021. Immunity. 54(8):1772-1787.e9. PubMed Jin RM, et al. 2017. J Immunol. 198:352. PubMed Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed Aktories P, et al. 2022. Cell Rep Methods. 2:100260. PubMed Zhang X, et al. 2021. Mol Cancer Res. 19:1076. PubMed Hernandez GE, et al. 2022. Nat Cardiovasc Res. 1:67. PubMed Bellomo A, et al. 2020. Immunity. 53(1):127-142.e7. PubMed Zhu YP et al. 2018. Cell reports. 24(9):2329-2341 . PubMed Fukasawa K, et al. 2016. Sci Rep. 6:30918. PubMed Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed Flak MB, et al. 2020. J Clin Invest. 130:359. PubMed Yáñez A et al. 2017. Immunity. 47(5):890-902 . PubMed
RRID: AB_2562667 (BioLegend Cat. No. 135513)
Structure: Ig superfamily, 145 kD
Distribution: Monocytes/macrophages, peritoneal exudate cells, plasmacytoid and conventional dendritic cells, osteoclasts
Function: Growth factor receptor, tyrosine kinase
Ligand/Receptor: Macrophage colony stimulating factor (M-CSF), IL-34
Cell Type: Dendritic cells, Macrophages, Monocytes, Osteoclasts
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: Sudo T, et al. 1995 Oncogene 11:2469. Murayama T, et al. 1999 Circulation 99:1740. Goswami S, et al. 2005 Cancer Res. 65:5278. Yu W, et al. 2008 J. Leuko. Biol. 84:852.
Gene ID: 12978
UniProt: View information about CD115 on UniProt.org
Clone: AFS98
Regulatory Status: RUO
Other Names: CSF-1R, M-CSFR, c-fms
Isotype: Rat IgG2a, κ
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.
Q: Why do I have a weak CD115 staining?
A: It has been reported that CD115 can be rapidly internalized, especially when samples are exposed to room temperature. Approximate 33% decrease in CD115 expression has been observed between 0 and 4 hours after sample collection, while overnight incubation of the cells results in complete CD115 downregulation.  Pre-treatment with EDTA and low temperatures (2 - 8°C) helps in maintaining surface expression of CD115. In addition, brief fixation of the cells with Fixation Buffer (Cat. No. 420801) for 10 minutes will block CD115 internalization.