Biolegend, 135809, PE/Cyanine7 anti-mouse CD88 (C5aR) Antibody, 25μg

CD88 is a single chain protein with seven membrane-spanning regions and a molecular weight of 43 kD. CD88 is expressed by neutrophils, eosinophils, and monocytes. There have been reports of CD88 expression in non-immune cells such as glial cells, cerebellar granule cells, cardiomyocytes, and vascular endothelial cells. CD88 is coupled to heterotrimeric G proteins such as G i , and after C5a binding, the signal transduced by CD88 results in the activation of PLCβ, PI-3 kinase, and PLA 2 , among other molecules, resulting in chemotaxis, granule enzyme release, and superoxide anion production.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: mC5aR transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications for the relevant formats include: blocking of C5a binding.
Application References(PubMed link indicates BioLegend citation): Godau J, et al. 2004. J. Immunol. 173:3437. Shushakova N, et al. 2002. J. Clin. Invest. 110:1823. Kim SH, et al. 2013. Int Immunol. 25:623. PubMed
Product Citations: Steitz AM, et al. 2023. iScience. 108401:26. PubMed Sharma S, et al. 2015. J Immunol. 194:5529. PubMed
RRID: AB_10900077 (BioLegend Cat. No. 135809) AB_10900812 (BioLegend Cat. No. 135810)
Structure: Single chain protein with seven membrane-spanning regions, 43 kD.
Distribution: Neutrophils, eosinophils, monocytes, glial cells, cerebellar granule cells, cardiomyocytes, and vascular endothelial cells.
Function: After C5a binding, the CD88 signal results in chemotaxis, granule enzyme release and superoxide anion production.
Interaction: CD88 is coupled to heterotrimeric G proteins such as Gi, The signal transduced by CD88 results in the activation of PLCβ, PI-3 kinase, and PLA2, among other molecules.
Bioactivity: Chemotaxis, degranulation.
Cell Type: Dendritic cells, Endothelial cells, Eosinophils, Monocytes, Neutrophils
Biology Area: Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family: CD Molecules
Antigen References: 1. Schraufstatter IU, et al. 2009. J. Immunol. 182:3827. 2. Ippel JH, et al. 2009. J. Biol. Chem. 284:12363. 3. Monk PN, et al. 2007. Br. J. Pharmacol. 152:429. 4. Griffin RS, et al. 2007. J. Neurosci. 27:8699. 5. Lee H, et al. 2006. Nat. Biotechnol. 24:1279. 6. Buck E and Wells JA, et al. 2005. P. Natl. Acad. Sci. USA 102:2719.
Gene ID: 12273
UniProt: View information about CD88 on UniProt.org
Clone: 20/70
Regulatory Status: RUO
Other Names: C5a ligand, C5a anaphylatoxin receptor, C5AR, C5R1, C5AR1
Isotype: Rat IgG2b, κ