Biolegend, 135909, Biotin anti-mouse CD140a Antibody, 25μg

Platelet-derived growth factor receptor-α (PDGFR-α), CD140a, is one of two receptors for platelet-derived growth factors (PDGFs) and binds to all isoforms of PDGFs: PDGF-AA, PDGF-AB, and PDGF-BB. PDGFRa is a receptor tyrosine kinase that forms homodimers or heterodimers on the surface upon ligand binding and phosphorylates substrates. PDGFRs consist of either homodimers of α/α, β/β, or heterodimers of α/β. PDGF receptors, α and β, are single glycoproteins with intracellular tyrosine kinase domain. Their ligand, PDGF, is a mitogen for connective tissue and glial cells. CD140a is expressed on embryonic tissues and mesenchymal-derived cells of adult mice. PDGF plays a role in wound healing and acts as a chemoattractant for fibroblasts, smooth muscle cells, glial cells, monocytes, and neutrophils.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse PDGFR-α-hIgG1 recombinant fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported (for relevant formats) applications include: Western Blot, blocking function2, and immunohistochemical staining of paraffin and frozen sections. The LEAF™ purified antibody is recommended for functional assays.
Application References(PubMed link indicates BioLegend citation): Takakura N, et al. 1996. J. Invest. Dermatol. 107:770. Liao C, et al. 2010. J. Clin. Invest. 120:242. (Block) Chen H, et al. 2015. ASN Neuro 8:7. PubMed. Miyawaki T, et al. 2004. J Neurosci. 24(37): 8124-34. (IHC-F)
Product Citations: Ye B, et al. 2022. Cell Mol Immunol. 19:619. PubMed Helms EJ, et al. 2021. Cancer Discov. Online ahead of print. PubMed Larsen SB, et al. 2021. Cell Stem Cell. 28:1758. PubMed Davidson S, et al. 2020. Cell Reports. 31(7):107628. PubMed Cheng HW, et al. 2019. Nat Commun. 10:1739. PubMed Adam RC et al. 2018. Cell stem cell. 22(3):398-413 . PubMed Horiguchi H, et al. 2019. Genes Dev. 33:1641. PubMed Laurin M, et al. 2019. Elife. 8. PubMed Miao Y et al. 2019. Cell. 177(5):1172-1186 . PubMed Kantelhardt S, et al. 2010. PLoS One. 5:e11323. PubMed Thomson CA, et al. 2018. J Immunol. 201:215. PubMed
RRID: AB_2043973 (BioLegend Cat. No. 135909) AB_2043974 (BioLegend Cat. No. 135910)
Structure: Alpha chain of the platelet-derived growth factor receptor, a receptor tyrosine kinase that forms homo- or hetero-dimers on the surface after ligand binding.
Distribution: Expressed on embryonic tissues and mesenchymal-derived cells of adult mouse.
Function: Play a role in wound healing and act as a chemoattractant for fibroblasts, smooth muscle cells, glial cells, monocytes and neutrophils.
Ligand/Receptor: PDGFs
Cell Type: Embryonic Stem Cells, Mesenchymal cells, Mesenchymal Stem Cells
Biology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Mukouyama YS, et al. 2006. Proc Natl Acad Sci USA. 103(5):1551 2. Miyawaki T, et al. 2004. J Neurosci. 24(37):8124 3. Takakura N, et al. 1997. J Histochem Cytochem. 45(6):883
Gene ID: 18595
UniProt: View information about CD140a on UniProt.org
Clone: APA5
Regulatory Status: RUO
Other Names: PDGF receptor-α, PDGFR-α
Isotype: Rat IgG2a, κ
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.