Biolegend, 136006, PE anti-mouse CD140b Antibody, 200μg

Platelet-derived growth factor receptor-β (PDGFR-β), CD140b, is one of two receptors for platelet-derived growth factors (PDGFs) and binds to all isoforms of PDGFs. PDGFRβ is a receptor tyrosine kinase that forms homodimers or heterodimers on the surface upon ligand binding and phosphorylates substrates. PDGFRs consist of either homodimers of α/α, β/β, or heterodimers of α/β. PDGF receptors, α and β, are single glycoproteins with intracellular tyrosine kinase domain. Their ligand, PDGF, is a mitogen for connective tissue and glial cells. CD140b is expressed on embryonic tissues and mesenchymal-derived cells adult mice. PDGF plays a role in wound healing and acts as a chemoattractant for fibroblasts, smooth muscle cells, glial cells, monocytes, and neutrophils.
200μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse PDGFR-β-hIgG1 recombinant fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported (for the relevant formats) applications include: Western blotting and blocking function2. The LEAF™ purified antibody is recommended for functional assays.
Application References(PubMed link indicates BioLegend citation): Sano H, et al. 2001. Circulation 103:2955. Sano H, et al. 2002. Am. J. Pathol. 161:135. (Block)
Product Citations: Zhang Q, et al. 2022. Nat Metab. 4:1055. PubMed Su Q, et al. 2021. iScience. 24:103233. PubMed Higashi AY, et al. 2019. J Am Soc Nephrol. 30:80. PubMed Sharma D, et al. 2022. Commun Biol. 5:479. PubMed Srivastava S, et al. 2019. Cancer Cell. 35:489. PubMed Jiang L, et al. 2022. Front Immunol. 12:730438. PubMed Hepler C et al. 2018. eLife. 7 pii: e39636. PubMed Li P, et al. 2022. EMBO Mol Med. 14:e14511. PubMed Habisch H, et al. 2008. Cytotherapy. 10:824. PubMed Shao M, et al. 2021. Cell Stem Cell. 28(4):685-701.e7. PubMed Joffin N, et al. 2021. Cell Stem Cell. 28(4):702-717.e8. PubMed Alonso-Herranz L, et al. 2020. Elife. 9:00. PubMed Shan B, et al. 2020. Nat Metab. 1332:2. PubMed Sheng D, et al. 2022. J Immunother Cancer. 10:. PubMed
RRID: AB_1953270 (BioLegend Cat. No. 136005) AB_1953271 (BioLegend Cat. No. 136006)
Structure: Beta chain of the platelet-derived growth factor receptor, a receptor tyrosine kinase that forms homo- or hetero-dimers on the surface after ligand binding.
Distribution: Expressed on embryonic tissues and mesenchymal-derived cells of adult mouse.
Function: Play a role in wound healing and act as a chemoattractant for fibroblasts, smooth muscle cells, glial cells, monocytes and neutrophils.
Ligand/Receptor: PDGFs
Cell Type: Embryonic Stem Cells, Mesenchymal Stem Cells
Biology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Soriano P, et al. 1994. Genes Dev. 8:1888 2. Takakura N, et al. 1996. J Invest Dermatol. 107:770 3. Yarden Y, et al. 1986. Nature 323:226
Gene ID: 18596
UniProt: View information about CD140b on UniProt.org
Clone: APB5
Regulatory Status: RUO
Other Names: PDGF receptor-β, PDGFR-β, platelet-derived growth factor receptor beta
Isotype: Rat IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.