Biolegend, 136205, FITC anti-mouse ESAM Antibody, 50μg

Endothelial cell-selective adhesion molecule (ESAM) is a 55-kD membrane protein composed of two extracellular Ig domains, a single transmembrane domain, and a cytoplasmic domain. ESAM is predominantly expressed at endothelial junctions and on platelets, participating in the migration of neutrophils through the vessel wall by influencing endothelial cell contacts. It impacts vascular permeability and extravasation process. Recently, it was reported that ESAM is a novel marker for murine hematopoietic stem cells (HSCs) in fetal liver. ESAM expression is correlated with HSC activity. The ESAM Hi population was highly enriched for multipotent myeloid-erythroid progenitors and primitive progenitors with lymphpoietic activity, and exclusively reconstituted long-term lymphohematopoiesis in lethally irradiated recipients.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse bEND.3 endothelial cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Application References(PubMed link indicates BioLegend citation): Nasdala I, et al. 2002. J. Bio. Chem. 277:16294
Product Citations: Crowell PD et al. 2019. Cell Rep. 28(6):1499-1510 . PubMed Sanmarco LM, et al. 2021. Nature. 590:473. PubMed Yamashita M, et al. 2019. Cell Stem Cell. 25:357. PubMed
RRID: AB_2044017 (BioLegend Cat. No. 136205)
Structure: A 55-kD membrane protein composed of two extracellular Ig domains, a single transmembrane domain, and a cytoplasmic domain.
Distribution: Expressed at endothelial junctions, platelets, and murine hematopoietic stem cells.
Function: It impacts vascular permeability and extravasation process. ESAM expression is involved in hematopoietic stem cell (HSC) activity.
Cell Type: Endothelial cells, Hematopoietic stem and progenitors, Platelets
Biology Area: Immunology, Innate Immunity
Molecular Family: Adhesion Molecules
Antigen References: 1. Wegmann F, et al. 2004. Exp Cell Res. 300:121 2. Ooi LAG, et al. 2008. Stem Cells. 27:653 3. Yokota T, et al. 2009. Blood 113(13):2871. Phosphate-buffered solution pH 7.2 containing 0.09% sodium azide.
Gene ID: 69524
UniProt: View information about ESAM on UniProt.org
Clone: 1G8/ESAM
Regulatory Status: RUO
Other Names: Endothelial cell-selective adhesion molecule
Isotype: Rat IgG2a, κ