Biolegend, 136405, APC anti-mouse CD309 (VEGFR2, Flk-1) Antibody, 25μg

CD309 is also known as vascular endothelial growth factor receptor 2 (VEGFR2) and fetal liver kinase-1 (Flk-1). CD309 is a member of the tyrosine protein kinase family that contains a single pass transmembrane receptor with a protein kinase domain and seven immunoglobulin-like domains in the extracellular region. CD309 is expressed at high levels in adult heart, lung, kidney, brain, and skeletal muscle. It's a receptor for VEGF or VEGFC, and plays an important role in the development of vascular endothelial cells, hematopoietic cells, and vascular permeability.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Murine Flk1 fused to hIgG Fc
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application Notes: Avas12 recognizes a different epitope than clone 89B3A5. Additional reported applications (for the relevant formats) include: Western blotting1 and immunohistochemical staining of paraformaldehyde-fixed frozen sections2.
Application References(PubMed link indicates BioLegend citation): Kataoka H, et al. 1997. Dev. Growth Differ. 39:729. (WB) Ishitobi H, et al. 2010. Exp. Anim. 59:615. (IHC-F)
Product Citations: Shao Y, et al. 2019. Diabetes. 68:2131. PubMed Vandoorne K, et al. 2018. Circ Res. 123:415. PubMed Beazley–Long N, et al. 2018. Brain Behav Immun. 3.117361111. PubMed Wang T, et al. 2021. EBioMedicine. 73:103632. PubMed Singhal P, et al. 2016. Proc Natl Acad Sci U S A. 113: 122 - 127. PubMed Shao Y, et al. 2019. Stem Cells. 37:1331. PubMed Rossi G, et al. 2020. Cell Stem Cell. 28(2):230-240.e6. PubMed Ishibashi R, et al. 2016. J Biol Chem. 291: 3333 - 3345. PubMed
RRID: AB_2044066 (BioLegend Cat. No. 136405) AB_2044067 (BioLegend Cat. No. 136406)
Structure: A receptor protein tyrosine kinase closely related to CD117 (c-kit) and CD140a (PDGF receptor α chain) of the immunoglobulin superfamily
Distribution: Expressed at high levels in adult heart, lung, kidney, brain, and skeletal muscle
Function: Plays an important role in the development of vascular endothelial cells, hematopoietic cells, and vascular permeability
Ligand/Receptor: Vascular endothelial growth factor (VEGF)
Biology Area: Angiogenesis, Cell Biology, Immunology
Molecular Family: Adhesion Molecules, CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Kaburn N, et al. 1997. Development. 124:2039 2. Patterson C, et al. 1995. J. Bio. Chem. 270:23111 3. Nishikawa SI, et al. 1998. Immunity 8 (6):761 4. Shalaby F, et al. 1997. Cell. 89 (6):981
Gene ID: 16542
UniProt: View information about CD309 on UniProt.org
Clone: Avas12
Regulatory Status: RUO
Other Names: Fetal liver kinase 1 (Flk-1), VEGF receptor-2, VEGF-R2, Ly-73, KDR
Isotype: Rat IgG2a, κ