Biolegend, 136511, PerCP/Cyanine5.5 anti-mouse CD93 (AA4.1, early B lineage) Antibody, 25μg

CD93 is a 130-140kD C-type lectin like type I transmembrane protein, also known as complement component 1, q subcomponent (C1qR1), C1qRp collectin receptor (C1qRp), or AA4 antigen. It is a receptor expressed on immature B lymphocytes, hematopoietic progenitors and stem cells in adult bone marrow, fetal liver, embryonic yolk sac. CD93 expression level on splenic immature/transitional B cells is much lower than in bone marrow. It is reinduced during plasma cell differentiation and plays an important role in maintaining plasma cells in bone marrow niches. Immature dendritic cells express CD93 and down-regulate this molecule upon maturation, suggesting a role in uptake of particles by DC. It is also expressed on monocytes, macrophages, and endothelial cells. Macrophages from CD93 (-/-) mice had a significant phagocytic defect in the clearance of apoptotic cells in vivo , indicating CD93 may contribute to the in vivo clearance of dying cells. Binding of CD93 to C1q remains controversial.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Pre-B lymphoma 70Z/3
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application References(PubMed link indicates BioLegend citation): McKearn JP, et al. 1984. J. Immunol. 132:332 Hanihara-Tatsuzaawa F, et al. 2014. J Biol Chem. 289:30925. PubMed
Product Citations: Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed Philpott J, et al. 2022. Immunohorizons. 6:366. PubMed Avery DT, et al. 2018. J Exp Med. 215:2073. PubMed Khiew SH, et al. 2020. J Clin Invest. 130:3453. PubMed Simoni L, et al. 2020. Cell Rep. 33:108330. PubMed Kunishita Y, et al. 2020. Front Immunol. 11:98. PubMed
RRID: AB_10645333 (BioLegend Cat. No. 136511) AB_10642683 (BioLegend Cat. No. 136512)
Structure: A 130-140 kD C-type lectin like type I transmembrane protein, also known as complement component 1, q subcomponent (C1qR1), C1qRp collectin receptor (C1qRp), or AA4 antigen.
Distribution: Expressed during immature B lymphocytes, hematopoietic progenitors and stem cells in bone marrow, immature dendritic cells, monocytes, macrophages, and endothelial cells.
Function: CD93 plays an important role in maintaining of plasma cells in bone marrow niches. It may play a role in uptake of particles by DC and the in vivo clearance of dying cells by macrophages.
Cell Type: B cells, Dendritic cells, Endothelial cells, Hematopoietic stem and progenitors, Macrophages, Monocytes
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family: CD Molecules
Antigen References: 1. Steinberger P, et al. 2002. J. Leukoc. Biol. 71:133 2. Chevrier S, et al. 2009. Proc. Nat. Acad. Sci. U. S. A. 106:3895 3. Norsworthy PJ, et al. 2004. J. Immunol. 172:3406 4. Li YS, et al. 1996. Immunity 5:527 5. Szilvassy SJ, et al. 1993. Blood 81:2310
Gene ID: 17064
UniProt: View information about CD93 on UniProt.org
Clone: AA4.1
Regulatory Status: RUO
Other Names: AA4.1, C1qRp, Early B lineage antigen
Isotype: Rat IgG2b, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.