Biolegend, 137605, FITC anti-mouse CD335 (NKp46) Antibody, 25μg

CD335, also known as NKp46, is a single-pass type I membrane protein of 46 kD. It belongs to the natural cytotoxicity receptor (NCR) family and contains two Ig-like (immunoglobulin-like) domains. It's expression is restricted to NK cells and a subset of NKT cells; it's not expressed in CD1d-restricted NKT cells. CD335 is a receptor for viral hemagglutinins and heparan sulfate proteoglycans and is involved in NK cell activation.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: NKP46-IgG1 Fc fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections1,2 and in vitro activation of NK cells1.
Application References(PubMed link indicates BioLegend citation): Walzer T, et al. 2007. P. Natl. Acad. Sci. USA 104:3384. (FC, Activ) Walzer T, et al. 2007. Nat. Immunol. 8:1337. (FC, Activ) Guerriero JL, et al. 2011. J. Immunol. 186:3517. (IHC) PubMed
Product Citations: Calabrese DR, et al. 2020. J Clin Invest. . PubMed Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed Saha S, et al. 2023. Sci Rep. 13:4609. PubMed Chang D, et al. 2023. Front Immunol. 14:1105145. PubMed Pelletier A, et al. 2023. EMBO Rep. 24:e56156. PubMed Rabia E, et al. 2023. Front Immunol. 14:1168444. PubMed Zhang Z, et al. 2023. Aging Dis. 14:966. PubMed Zhao Z, et al. 2022. Adv Sci (Weinh). 9:e2201293. PubMed Huang J, et al. 2021. Immunity. 54(4):673-686.e4. PubMed Miller CM, et al. 2020. J Virol. 94:00:00. PubMed Shapiro MR, et al. 2020. Front Immunol. 1.972222222. PubMed Piranlioglu R, et al. 2019. Nat Commun. 10:1430. PubMed Ellis G, et al. 2015. EMBO Rep. 16: 1203-1218. PubMed Zhou Y, et al. 2020. Immunity. 52(2):357-373. PubMed He J, et al. 2020. Cell Reports. 29(9):2718-2730.e6.. PubMed Lissner MM, et al. 2020. Elife. 9:00. PubMed Chun E, et al. 2020. Immunity. 51(5):871-884.e6.. PubMed Li J, et al. 2018. Immunity. 49:178. PubMed Marcus A et al. 2018. Immunity. 49(4):754-763 . PubMed Sasaki T, et al. 2020. Cell Reports. 28(1):202-217.e7.. PubMed Liu W, et al. 2019. Epigenetics. 1.192361111. PubMed Zhu Y, et al. 2021. EMBO J. 40:e105320. PubMed Gubin MM, et al. 2018. Cell. 175:1014. PubMed Kawamura A, et al. 2016. Sci Rep. 6:19464. PubMed Martin MD, et al. 2020. Cell Rep. 31:107508. PubMed Wang T et al. 2018. Immunity. 49(3):504-514 . PubMed Bhatt D, et al. 2021. J Exp Med. 218:. PubMed Lam KC, et al. 2021. Cell. 184:5338. PubMed Natale CA, et al. 2018. Elife. 7. PubMed Srivastava S, et al. 2020. Cancer Cell. 39(2):193-208.e10. PubMed Ukidve A, et al. 2020. Proc Natl Acad Sci U S A. 117:17727. PubMed Burton OT, et al. 2018. Clin Exp Allergy. 48:288. PubMed Rivera CA, et al. 2022. Immunity. 55:129. PubMed
RRID: AB_2149150 (BioLegend Cat. No. 137605) AB_2298210 (BioLegend Cat. No. 137606)
Structure: Single-pass type I membrane protein, 46 kD; belongs to the natural cytotoxicity receptor (NCR) family; contains 2 Ig-like (immunoglobulin-like) domains
Distribution: Mature and immature NK cells, subset of NKT cells, but not on CD1d-restricted NKT cells
Function: NK cells activation
Ligand/Receptor: Viral hemagglutinins, heparan sulfate proteoglycans
Cell Type: NK cells, NKT cells
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules
Antigen References: 1. Colucci F and Cilio CM. 2010. Nat. Immunol. 125:60. 2. Caligiuri MA. 2008. Blood 112:461. 3. Colonna M. 2009. Immunity 31:15.
Gene ID: 17086
UniProt: View information about CD335 on UniProt.org
Clone: 29A1.4
Regulatory Status: RUO
Other Names: NKp46, NCR1
Isotype: Rat IgG2a, κ