Biolegend, 137648, PE/Cyanine5 anti-mouse CD335 (NKp46) Antibody, 100μg

CD335, also known as NKp46, is a single-pass type I membrane protein of 46 kD. It belongs to the natural cytotoxicity receptor (NCR) family and contains two Ig-like (immunoglobulin-like) domains. It's expression is restricted to NK cells and a subset of NKT cells; it's not expressed in CD1d-restricted NKT cells. CD335 is a receptor for viral hemagglutinins and heparan sulfate proteoglycans and is involved in NK cell activation.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: NKP46-IgG1 Fc fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Cyanine5 under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections1,2 and in vitro activation of NK cells1.
Application References(PubMed link indicates BioLegend citation): Walzer T, et al. 2007. P. Natl. Acad. Sci. USA 104:3384. (FC, Activ) Walzer T, et al. 2007. Nat. Immunol. 8:1337. (FC, Activ) Guerriero JL, et al. 2011. J. Immunol. 186:3517. (IHC) PubMed
Product Citations: Diamantopoulou Z, et al. 2022. Nature. 607:156. PubMed
RRID: AB_2894656 (BioLegend Cat. No. 137647) AB_2894656 (BioLegend Cat. No. 137648)
Structure: Single-pass type I membrane protein, 46 kD; belongs to the natural cytotoxicity receptor (NCR) family; contains 2 Ig-like (immunoglobulin-like) domains
Distribution: Mature and immature NK cells, subset of NKT cells, but not on CD1d-restricted NKT cells
Function: NK cells activation
Ligand/Receptor: Viral hemagglutinins, heparan sulfate proteoglycans
Cell Type: NK cells, NKT cells
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules
Antigen References: 1. Colucci F and Cilio CM. 2010. Nat. Immunol. 125:60. 2. Caligiuri MA. 2008. Blood 112:461. 3. Colonna M. 2009. Immunity 31:15.
Gene ID: 17086
UniProt: View information about CD335 on UniProt.org
Clone: 29A1.4
Regulatory Status: RUO
Other Names: NKp46, NCR1
Isotype: Rat IgG2a, κ