Biolegend, 137711, PerCP/Cyanine5.5 anti-mouse TCR Vγ2 Antibody, 25μg

T cell receptor (TCR) Vγ2 bearing T lymphocytes make up a significant proportion of γδ TCR cells in late fetal and adult peripheral lymphoid tissues. TCR γδ T cells may play a role in immunological surveillance for stress-induced self-antigens. The frequency of Vγ2 expression in different strains varies from 12% to 54% in the TCR γδ repertoire. Variations in the levels of Vγ2 + cells are not associated with MHC haplotype. Clone UC3-10A6 reacts with the Vγ2 segment as designated by the Garman nomenclature. 1 Mouse TCR Vγ2 is also known as TCR Vγ4 under the Heilig & Tonegawa nomenclature system. Several TCR γ and δ chain nomenclature systems exist. In order to consolidate the various nomenclature systems, we have created charts for corresponding names across some of the most popular naming methods. Click here to see charts .
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Armenian Hamster
Immunogen: G8 mouse T cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application References(PubMed link indicates BioLegend citation): Dent AL, et al. 1990. Nature 343:714. Kelly KA, et al. 1993. Int. Immunol. 5:331. Sperling AI, et al. 1992. J. Immunol. 149:3200. Sperling AI, et al. 1997. J. Immunol. 159:86.
RRID: AB_2814039 (BioLegend Cat. No. 137711) AB_2814040 (BioLegend Cat. No. 137712)
Structure: A member of Ig superfamily, associated with TCR δ chain and CD3 complex.
Distribution: T cell subset in fetal and adult peripheral lymphoid tissues and thymus.
Function: Might play a role in a previously unrecognized form of immunological surveillance for stress-induced self-antigens.
Ligand/Receptor: Antigens
Cell Type: T cells
Biology Area: Adaptive Immunity, Immunology
Molecular Family: TCRs
Antigen References: 1. Allison JP, et al. 1991. Annu. Rev. Immunol. 9:679. 2. O’Brien RL, et al. 2000. J. Immunol. 165:6472.
Gene ID: 110067
UniProt: View information about TCR Vgamma2 on UniProt.org
Clone: UC3-10A6
Regulatory Status: RUO
Other Names: Vγ2 T-cell receptor, T cell receptor gamma 2, TCR Vγ4 (Tonegawa nomenclature)
Isotype: Armenian Hamster IgG
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.