Biolegend, 138001, Purified anti-mouse CD144 (VE-cadherin) Antibody, 50μg

CD144, also known as vascular endothelial-cadherin (VE-cadherin), is a 120 kD member of the type II Cadherin family.  It is an endothelial specific hemophilic adhesion molecule involved in endothelial cell survival, migration, contact-dependent growth inhibition, and homophilic adhesion.  VE-cadherin is essential for maintaining the integrity of the endothelial barrier in vivo .
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: VE-cadherin-Ig fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested WB, ICC, Block - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. It is also recommended using EDTA-based solutions for dissociating attachment-dependent cell lines.
Application Notes: Clone BV13 recognizes an epitope between aa 45 and 56, and has a binding affinity of 5-15 nM5. Additional reported applications (for relevant formats) include: Western blotting1, blocking of cell interactions in vivo1, and immunofluorescence microscopy staining4.
Application References(PubMed link indicates BioLegend citation): Corada M, et al. 1999. P. Natl. Acad. Sci. USA 96:9815. (WB, Block) Liao F, et al. 2000. Cancer Res. 60:6805. (FC) Crosby CV, et al. 2005. Blood 105:2771. (FC) Liao F, et al. 2002. Cancer Res. 62:2567. (IF) May C, et al. 2005. Blood 105:4337. (epitope)
Product Citations: Gómez-Salinero JM, et al. 2022. Nat Cardiovasc Res. 1:882. PubMed Long Y, et al. 2022. Front Pharmacol. 13:1039103. PubMed Harrison CB, et al. 2020. Arterioscler Thromb Vasc Biol. ATVBAHA120315322:. PubMed Piao W, et al. 2020. Cell Reports. 30(4):1052-1062.e5.. PubMed Poulos MG, et al. 2017. J Clin Invest. 127:4163. PubMed Sakurai Y, et al. 2018. Mol Ther Oncolytics. 11:102. PubMed Wakabayashi T et al. 2018. Cell stem cell. 22(3):384-397 . PubMed Ecker BL, et al. 2019. Cancer Discov. 0.431944444. PubMed
RRID: AB_10570497 (BioLegend Cat. No. 138001) AB_10569559 (BioLegend Cat. No. 138002)
Structure: A 120 kD member of type II Cadherin family
Distribution: Endothelial cells
Function: Involved in endothelial cell survival, migration, contact-dependent growth inhibition, and hemophilic adhesion
Ligand/Receptor: CD144
Cell Type: Endothelial cells, Mesenchymal Stem Cells
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Allport JR, et al. 2002. J. Leukocyte Biol. 71:821. 2. Hirashima M, et al. 2009. Blood 93:1253. 3. Matsuyoshi N, et al. 1997. Proc. Assoc. Am. Physicians 109:362. 4. Matsumura K, et al. 2003. Blood 101:1367. 5. Hirashima M, et al. 2009. Blood 101:2261. 6. Gotsch U, et al. 1997. J. Cell Sci. 110:583. 7. Kataoka H, et al. 1997. Dev. Growth Differ. 39:729.
Gene ID: 12562
UniProt: View information about CD144 on UniProt.org
Clone: BV13
Regulatory Status: RUO
Other Names: Vascular endothelial-cadherin
Isotype: Rat IgG1, κ