Biolegend, 138011, APC anti-mouse CD144 (VE-cadherin) Antibody, 25μg

CD144, also known as vascular endothelial-cadherin (VE-cadherin), is a 120 kD member of the type II Cadherin family.  It is an endothelial specific hemophilic adhesion molecule involved in endothelial cell survival, migration, contact-dependent growth inhibition, and homophilic adhesion.  VE-cadherin is essential for maintaining the integrity of the endothelial barrier in vivo .
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: VE-cadherin-Ig fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. It is also recommended using EDTA-based solutions for dissociating attachment-dependent cell lines.
Excitation Laser: Red Laser (633 nm)
Application Notes: Clone BV13 recognizes an epitope between aa 45 and 56, and has a binding affinity of 5-15 nM5. Additional reported applications (for relevant formats) include: Western blotting1, blocking of cell interactions in vivo1, and immunofluorescence microscopy staining4.
Application References(PubMed link indicates BioLegend citation): Corada M, et al. 1999. P. Natl. Acad. Sci. USA 96:9815. (WB, Block) Liao F, et al. 2000. Cancer Res. 60:6805. (FC) Crosby CV, et al. 2005. Blood 105:2771. (FC) Liao F, et al. 2002. Cancer Res. 62:2567. (IF) May C, et al. 2005. Blood 105:4337. (epitope)
Product Citations: Jurkin J, et al. 2016. J Allergy Clin Immunol. S0091-6749(16)31121-6. PubMed Bibli SI, et al. 2020. Redox Biol. 28:101379. PubMed Spitzer D, et al. 2023. Nat Protoc. 18:1510. PubMed Liu D, et al. 2021. JCI Insight. 6:. PubMed Dieterich LC, et al. 2019. International Journal of Cancer. 145(10):2804-2815. PubMed Ni F, et al. 2019. Cell Stem Cell. 24:608. PubMed Park M, et al. 2021. BMB Rep. 54:470. PubMed Geis T, et al. 2014. Exp Cell Res. 331:46. PubMed Gomez I, et al. 2020. Nat Commun. 11:214. PubMed Ye P, et al. 2022. Front Cardiovasc Med. 8:810477. PubMed Ballesteros I, et al. 2020. Cell. 183(5):1282-1297.e18. PubMed Singhal P, et al. 2016. Proc Natl Acad Sci U S A. 113: 122 - 127. PubMed
RRID: AB_10679039 (BioLegend Cat. No. 138011) AB_10680420 (BioLegend Cat. No. 138012)
Structure: A 120 kD member of type II Cadherin family
Distribution: Endothelial cells
Function: Involved in endothelial cell survival, migration, contact-dependent growth inhibition, and hemophilic adhesion
Ligand/Receptor: CD144
Cell Type: Endothelial cells, Mesenchymal Stem Cells
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Allport JR, et al. 2002. J. Leukocyte Biol. 71:821. 2. Hirashima M, et al. 2009. Blood 93:1253. 3. Matsuyoshi N, et al. 1997. Proc. Assoc. Am. Physicians 109:362. 4. Matsumura K, et al. 2003. Blood 101:1367. 5. Hirashima M, et al. 2009. Blood 101:2261. 6. Gotsch U, et al. 1997. J. Cell Sci. 110:583. 7. Kataoka H, et al. 1997. Dev. Growth Differ. 39:729.
Gene ID: 12562
UniProt: View information about CD144 on UniProt.org
Clone: BV13
Regulatory Status: RUO
Other Names: Vascular endothelial-cadherin
Isotype: Rat IgG1, κ