Biolegend, 138605, FITC anti-mouse Ly-6D Antibody, 25μg

Lymphocyte antigen 6D (Ly-6D), also known as thymocyte B-cell antigen (ThB), is a 16 kD GPI-linked cell surface glycoprotein. It belongs to the Ly6 gene family and contains one urokinase receptor (uPAR) /Ly6 domain. Ly-6D is involved in cell-cell adhesion and signal transduction. Ly-6D marks the earliest stage of B cell lineage commitment from the common lymphoid progenitors (CLP). It is expressed on B cells, immature thymocytes, and plasmacytoid dendritic cells.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: BALB/c mouse plasmacytoma MOPC-104E
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Application References(PubMed link indicates BioLegend citation): Eckhardt LA and Herzenberg LA. 1980. Immunogenetics 11:275. (FC)
Product Citations: Haensel D, et al. 2023. Nat Commun. 14:2685. PubMed Haensel D, et al. 2022. Nat Commun. 13:7520. PubMed Weiner AI 2022. Cell Reports. 41(11):111805. PubMed Kurkewich J, et al. 2016. PLoS One. 11: 0161468. PubMed Janela B, et al. 2019. Immunity. 50:1069. PubMed Philip E Boulais et al. 2018. Immunity. 49(4):627-639 . PubMed
RRID: AB_11203901 (BioLegend Cat. No. 138605) AB_11203888 (BioLegend Cat. No. 138606)
Structure: GPI-linked cell-surface glycoprotein, molecular weight 16 kD, belongs to the Ly6 gene family, contains one urokinase receptor (uPAR) /Ly6 domain
Distribution: B cells, B cell-biased lymphoid progenitor (BLP), thymocytes, plasmacytoid dendritic cells
Function: Cell-cell adhesion and signal transduction
Cell Type: B cells, Dendritic cells, Thymocytes
Biology Area: Cell Adhesion, Cell Biology, Immunology, Signal Transduction
Molecular Family: Adhesion Molecules
Antigen References: 1. Inlay MA, et al. 2009. Genes Dev. 23:2376. 2. Gumley TP, et al. 1995. Immunol. Cell Biol. 73:277. 3. Mansson R, et al. 2010. Blood 115:2601. 4. Reese JT, et al. 2001. Dev. Immunol. 8:107.
Gene ID: 17068
UniProt: View information about Ly-6D on UniProt.org
Clone: 49-H4
Regulatory Status: RUO
Other Names: Lymphocyte antigen 6D, Thymocyte B-cell antigen, ThB
Isotype: Rat IgG2c, κ